一株高效产油脂真菌的选育及其油脂成分分析  

Screening of High Lipid-producing Fungus and Analysis of Composition of the Lipid

作　　者：马占军[1,2] 蔡冠竟[2,3] 杨旭俊[2,3] 郑天凌[2,3] 

机构地区：[1]厦门大学化学化工学院 [2]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室 [3]近海海洋环境科学国家重点实验室(厦门大学),福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2012年第6期1079-1084,共6页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金重点项目(40930847);面上项目(31070442);福建省自然科学基金项目(2012J01150)

摘　　要：以早期从海洋环境中分离得到的38株海洋真菌为材料,采用苏丹黑染色法初筛,摇瓶法复筛,得到1株油脂产率相对较高(27.96%)的菌株MD2,经鉴定,该菌株为粘红酵母(Rhodotorula glutinis).再以MD2为出发菌株,经紫外诱变、亚硝基胍诱变和紫外-亚硝基胍复合诱变,最终得到突变菌株MD2-1;采用酸热法提取粘红酵母MD2-1的菌体油脂,测定了其产脂能力,并采用气相色谱-质谱联用法(GC-MS)测定其脂肪酸组成.结果表明:MD2-1的产脂能力较强,油脂质量达菌体干质量的45.32%,较MD2提高17.36%;MD2-1菌体油脂中检测出9种脂肪酸,其脂肪酸组成与植物油近似,其中10-十八(碳)烯酸占脂肪酸总量的71.53%,为10-十八(碳)烯酸工业化生产提供菌种资源,为其规模化生产奠定基础.Thirty-eight marine fungi strains were isolated from marine environment and subjected to preliminary screening with Su dan Black dye and further screening by shake-flask culture. As a result, strain MD2 was found to have the highest lipid content of 27.96% and identified as Rhodotorula glutinis. The strain was mutagenized respectively with UV light, nitrosoguanidine （NTG） and their combination, obtaining the mutant MD2-1. The lipid was extracted from the strain Rhodotorula glutinis MD2-1 by acid- heat extracting methods, and its fatty acids was analyzed by GC-MS. The results showed that R. glutinis MD2-1exhibited strong a- bility to produce lipid, and the total amount of lipid represented an average of 45.32% of the dry strain weight. The fatty acid com- position of the lipid from R. glutinis MD2-1 was similar to vegetable oil and fat, and the 10-octadecenoic acid accounted for 71.53 % of the total fatty acids, so this research made a major significance： provided seed resources for industrial production of 10-octadeceno ic acid and laid the foundation for its large-scale production.

关 键 词：海洋真菌 诱变 油脂 脂肪酸 组成分析 

分 类 号：Q939.9[生物学—微生物学]