小鼠2-细胞期胚胎细胞电融合的研究  

Electrofusion of Mouse Two-Cell Embryonic Cells

作　　者：陈乃清[1] 赵浩斌[2] 

机构地区：[1]武汉大学生命科学学院,武汉430072 [2]湖北省农业科学院畜牧兽医研究所,武汉430209

出　　处：《武汉大学学报（自然科学版）》2000年第2期243-245,共3页Journal of Wuhan University(Natural Science Edition)

基　　金：国家自然科学基金资助项目!( 3 92 70 5 17)

摘　　要：为了探索核移植克隆动物技术中的细胞融合参数 ,进行了小鼠 2 -细胞期胚胎细胞的电融合试验 .直流电场强度为 2 k V/ cm,脉冲时程 40 μs时 ,效果较好 ,可以获得 70 .5 %的融合率和 6 4.5 %的发育率 ,进一步提高电场强度 ,裂解死亡率也明显提高 ;采用非电解质溶液 (甘露醇 )作为融合液时 ,胚胎细胞的融合率与融合胚的发育均优于电解质融合液 (DPBS) ;在直流电脉冲之前 ,施加交流电脉冲是必要的 ,可以显著提高融合率 (P<0 .0 5 ) .The techniques on electrofusion of two\|cell embryonic cells were systematically studied. When direct current field strength were 2 kV/cm and pulse duration was 40 μs, the fusion rate and development rate of the fused embryo accounted for 70.5% and 64.5%, respectively. Comparison of nonelectrolyte and electrolyte electrofusion media showed that the nonelectrolyte was better than electrolyte for fusion rate and development rate. In addition, it has been confirmed that the alternating current pulse antedated direct current should be necessary for the embryonic cells to be fused, with the significantly increased fusion rate ( P <0.05).

关 键 词：电融合 胚胎细胞 小鼠 2-细胞期 胚胎发育 

分 类 号：Q492.6[生物学—生理学] S852.1[农业科学—基础兽医学]