LC-MS/MS方法测定人血浆中盐酸非那吡啶的浓度  

Determination of phenazopyridine hydrochloride in human plasma by LC-MS/MS

作　　者：李天云[1] 魏敏吉[1] 

机构地区：[1]北京大学第一医院临床药理研究所,北京100034

出　　处：《中国临床药理学杂志》2012年第11期854-856,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立LC-MS/MS法测定人血浆中盐酸非那吡啶浓度。方法色谱柱为Capcell PAK C18,流动相为甲醇-10 mmol乙酸铵(85∶15,pH=4),流速为0.15 mL.min-1,电喷雾离子源(ESI),正离子方式检测,多反应监测(MRM)。结果盐酸非那吡啶的线性范围为0.2～50.0 ng.mL-1,日内、间差RSD均≤11.5%,提取回收率为≥60.2%。结论该方法灵敏、准确、快速,可用于人血浆盐酸非那吡啶浓度的测定。Objective To establish a new LC–MS/MS method for the determination of phenazopyridine hydrochloride in human plasma.Methods The analysis column was conducted with Capcell PAK C18.methanol-ammonium acetate（5∶15） was served as mobile phase.The flow rate was 0.15 mL·min-1.Detection was performed on a tandem mass spectrometry with electrospray ionization source.Multiple reactions monitoring mode was used to quantify phenazopyridine hydrochloride.Results The linear calibration curves were obtained in the range of 0.2-50.0 ng·mL-1for phenazopyridine hydrochloride.The extraction recovery for phenazopyridine hydrochloride were≥60.2%.The intra and inter day precision were≤11.5%.Conclusion The method is accurate,sensitive,and convenient,suitable for therapeutic drug monitoring and pharmacokinetic study of phenazopyridine hydrochloride.

关 键 词：液质联用 盐酸非那吡啶 血浆浓度 

分 类 号：R969.1[医药卫生—药理学] R983[医药卫生—药学]