隆林山羊肌细胞生成素基因的序列分析与结构预测  

Sequence Analysis and Structure Prediction of MyoG Gene in Longlin Goat

作　　者：赵子贵[1] 黄晨[1] 宋少锐[1] 曹植植[1] 郭亚芬[1] 兰干球[1] 杨膺白[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《中国畜牧兽医》2012年第11期17-21,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广西科技厅科学研究与技术开发计划项目(桂科攻10100007-5)

摘　　要：为了丰富优良地方品种隆林山羊的肌细胞生成素(myogenin,MyoG)基因遗传学基础信息,并探究其序列结构及其对山羊肌肉的发育分化调控机理。本研究设计1对特异性引物,采用PCR和克隆技术成功获得隆林山羊MyoG基因2419bp长的DNA序列。结果表明,该基因包括3个外显子、2个内含子、部分5′UTR和3′UTR区,编码区DNA序列长675bp,共编码224个氨基酸,该氨基酸序列无信号肽序列和跨膜结构。经同源性分析可知,隆林山羊MyoG编码区核苷酸序列及其编码的氨基酸序列与其他物种比对结果和氨基酸系统进化树的聚类结果相符,均显示隆林山羊与哺乳动物中的绵羊、牛和野猪的亲缘关系最近,氨基酸同源性达到95%以上。因此隆林山羊MyoG基因在哺乳动物中具有较高的保守性,而MyoG基因的克隆、序列分析和结构预测将为今后该基因的表达与调控、肉质改良、山羊开发利用等研究提供了更充分的分子生物学基础信息和理论依据。To enrich the basical genetic information and explore the sequence structure and the regulation mechanism of muscle differentiation in local excellent variety Longlin goat MyoG gene.A pair of primers was designed to amplify the sequence of MyoG gene from Longlin goat genome.The results showed that the sequence of MyoG gene with 2419 bp consisted of 3 exon,2 intron and some of 5′and 3′untranslated regions.With 675 bp coding sequences,encoding a total of 224 amino acids,and the peptide chain had not signal peptide and transmembrane structure.Homology analysis showed,comparing the coding sequences and coding amino acids of Longlin goat MyoG with other species,the cluster result was similar to the phylogenetic tree.Both showed that Longlin goat and sheep,cattle,wild boar were closest relative,the homology of amino acid sequences between them was above 95%.So the mammalian MyoG gene was conservative in evolution,and cloning,sequence analysis and structure prediction of Longlin goat MyoG gene provided the biological information and theoretical basis for researching the gene expression and regulation,improving the muscle quality and developing goats breed.

关 键 词：隆林山羊 MYOG基因 序列分析 结构预测 

分 类 号：Q78[生物学—分子生物学]