冷冻速率和解冻温度对猪精液冷冻效果的影响  

Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen

作　　者：梁鸿斌[1] 姚学军[2] 李维华[1] 邵慧[1] 朱金清 邬纯鸿[1] 倪和民[4] 刘云海[4] 郭勇[4] 

机构地区：[1]北京奶牛中心,北京100192 [2]北京市昌平区动物疫病预防控制中心,北京102200 [3]北京市昌平区种猪场,北京102212 [4]北京农学院动物科学技术学院,北京102206

出　　处：《中国畜牧兽医》2012年第11期157-160,共4页China Animal Husbandry & Veterinary Medicine

基　　金：十一五国家科技支撑计划项目“荣昌猪资源保护体系及关键技术研究”(2007BAD51B01-1-2)

摘　　要：为优化冷冻和解冻方法,提高冷冻效果,本试验比较了不同冷冻速率(-100℃10min、-120℃10min、-140℃10min)和不同解冻温度(37℃30s、45℃30s、52℃30s、60℃30s)对猪精液冷冻效果的影响。结果表明,采用-120℃熏蒸10min,解冻后精子活力为0.36,质膜完整率和顶体完整率也优于其他2组,且差异显著(P<0.05)。采用37℃30s方法解冻,精子活力、质膜完整率显著高于其他3组,顶体完整率也高于其他3组,但差异不显著(P>0.05),其畸形率最低和60℃30s组差异明显(P<0.05),但与45℃30s组和52℃30s组差异不显著(P>0.05)。因此,采用-120℃平衡10min冷冻,37℃30s水浴解冻方法更为适合0.25mL细管猪冻精解冻。For improving the freezing and de-freezing methods for boar semen further,it was aimed to investigate the effect of different freezing frequency（-100 ℃ 10 min,-120 ℃ 10 min,-140 ℃ 10 min） and thawing temperature（37 ℃ 30 s,45 ℃ 30 s,52 ℃ 30 s,60 ℃ 30 s） on cryopreservation of boar semen.The results showed that the sperm motility,plasma integrity,normal acrosome rate by freezing treatment with-120 ℃ 10 min,were all significantly higher than those of the other three groups respectively（P0.05）.The sperm motility,plasma integrity,normal acrosome rate by thawing treatment with 37 ℃ 30 s,were not significantly higher than those of the other three groups respectively（P0.05）,but the difference of the sperm abnormality in the treatment with 60 ℃ 30 s was significant（P0.05）,but with 45 ℃ 30 s and 52 ℃ 30 s were not significant（P0.05）.Therefore,it could be concluded that the combination of freezing by-120 ℃ 10 min and thawing by 37 ℃ 30 s were appropriate to frozen-thawed 0.25 mL pipettes of boar semen.

关 键 词：猪 精液冷冻 细管 冷冻速率 体外受精 

分 类 号：S828.34[农业科学—畜牧学]