复合氰基丙烯酸酯-骨形态发生蛋白生物胶预防大鼠牙槽嵴萎缩的实验研究  

A study on the compound bone morphogenetic protein with chitosan and cyanoacrylate on preventive absorption of rat's alveolar ridge

作　　者：张贇[1,2] 谢富强 王新[1,2] 胡朝昶[1,2] 孙健[1] 

机构地区：[1]兰州大学第二医院口腔科,兰州730030 [2]兰州大学口腔医学院,兰州730000

出　　处：《华西口腔医学杂志》2012年第6期584-588,593,共6页West China Journal of Stomatology

摘　　要：目的制备复合氰基丙烯酸酯-骨形态发生蛋白(BMP)生物胶,并植入大鼠拔牙窝观察其预防牙槽嵴萎缩的效果。方法选用Wistar大鼠54只,随机分为3组:实验组(复合氰基丙烯酸酯-BMP生物胶组)、对照组(壳聚糖温敏性胶体组)、空白对照组。拔除大鼠下颌切牙后,实验组和对照组在牙槽窝内即刻植入复合氰基丙烯酸酯-BMP生物胶、单纯壳聚糖温敏性胶体,空白对照组则不植入任何材料。3组动物在植入后3、6、9周处死,分离切取下颌骨,用高频钼靶扫描仪获得图像信息,使用Image-Pro Plus软件处理图像,得到剩余牙槽嵴相对高度和牙槽窝相对光密度,组织学观察牙槽窝内的成骨情况。结果术后3、6、9周,实验组的剩余牙槽嵴相对高度和牙槽窝相对光密度均高于对照组和空白对照组(P<0.05);组织学观察显示实验组牙槽窝成骨多于对照组和空白对照组。结论复合氰基丙烯酸酯-BMP生物胶可以用于预防拔牙术后剩余牙槽嵴萎缩,其临床应用前景广阔。Objective To evaluate the effectiveness and feasibility of compound bone morphogenetic protein （BMP） with chitosan and cyanoacrylate on preventive absorption of rat＇s alveolar ridge. Methods Fifty-four Wistar rats were divided randomly into three groups： Experimental group （compound BMP with chitosan and cyanoacrylate group）, control group（chitosan composite group） and blank group. After rat lower incisors were extracted, compound BMP with chitosan and cyanoacrylate were implanted into experimental group rat＇s residual alveolar fossa, control group implanted with chitosan, blank group left empty. Animals were sacrificed after 3, 6, 9 weeks. X-ray examination, Image-Pro Plus analysis and pathologic observation were evaluated. Results The relative length values of residual alveolar ridge and optical density test in experimental group were all greater than those of control group and blank group at 3, 6, 9 weeks after operation （P〈0.05）. Light microscopy showed that new bone formations in experimental group were better than those in control group and blank group at 3, 6, 9 weeks after implantation. Conclusion Compound BMP with chi- tosan and cyanoacrylate can be used to prevent the absorption of residual alveolar ridge and have more clinical predications.

关 键 词：骨形态发生蛋白 氰基丙烯酸酯 壳聚糖 牙槽嵴萎缩 

分 类 号：R318.08[医药卫生—生物医学工程]