甘草酸对酸性环境中变异链球菌生长影响的体外研究  

Effect of glycyrrhizic acid on growth of Streptococcus mutans under acid environment in vitro

作　　者：张凌楠[1] 何永红[2] 张菲菲[3] 田婷玉[1] 宋伟[1] 万呼春[1] 

机构地区：[1]四川大学华西口腔医院特诊科 [2]口腔疾病研究国家重点实验室,四川大学,成都610041 [3]泰安市口腔医院牙体牙髓科,泰安271000

出　　处：《华西口腔医学杂志》2012年第6期594-597,602,共5页West China Journal of Stomatology

摘　　要：目的研究酸性环境中甘草酸对体外培养变异链球菌生长的影响。方法在pH 7.0、pH 5.5和pH 4.0环境中培养变异链球菌,同时在培养基中加入3种浓度(0.78、1.57、3.13 mg·mL-1)的甘草酸,37℃下厌氧培养24 h后,测定各组的菌落计数和菌悬液吸光度值,分别以阴性对照组和pH 7.0组细菌生存率为对照,计算各组细菌的生存率。结果以阴性对照组为对照,0.78、1.57和3.13 mg·mL-1甘草酸组在pH 5.5环境时细菌生存率为60.96%、60.27%和45.58%,在pH 4.0时细菌生存率为68.75%、53.12%和45.83%。以pH 7.0组为对照,0.78、1.57和3.13 mg·mL-1甘草酸组在pH 5.5和pH 4.0时细菌生存率分别为52.25%和39.05%、74.39%和43.11%、86.38%和55.30%。结论在酸性环境中,甘草酸能够抑制体外变异链球菌的生长,环境酸度降低可使甘草酸的抑菌作用增强。Objective To investigate the antibacterial activity of glycyrrhizic acid（GA） against Streptococcus mutans （S.mutans） under acidic environment in vitro. Methods Working culture were prepared by inoculation of S.mutans into TPY broth followed by static incubation under anaerobic condition at 37 ~C for 24 h. TPY broth was supplemented with three kinds density of GA（0.78, 1.57, 3.13mg＇mL-l）, whose acidity was regulated to pH7.0, pH5.5 and pH4.0. And the group of pH 7.0 was used as negative control. The growth of S. mutans was measured by A^0 of bacteria suspen- sion and counting colony forming unit （CFU）. In addition, the survival rate of S. mutans was calculated. Results In pH 5.5 groups, the survival rates of 0.78, 1.57 and 3.13 mg.mL-t GA groups were 60.96%, 60.27% and 45.58%, res- pectively, and in pH4.0 groups, the survival rates were 68.75%, 53.12% and 45.83%. In 0.78, 1.57 and 3.13 mg＇mL-1 GA groups, the survival rates of pH 5.5 and pH 4.0 were 52.25% and 39.05%, 74.39% and 43.11%, 86.38% and 55.30%, respectively. Condlmion GA could inhibit the growth of S.mutans under acidic environment, which the effect is im- proved as the acidity increased.

关 键 词：甘草酸 变异链球菌 耐酸性 

分 类 号：R285[医药卫生—中药学]