中华蜜蜂dynactin p62基因的克隆及不同发育阶段表达分析  

Cloning and developmental expression of dynactin p62 gene in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)

作　　者：曾晶[1] 潘其忠[1] 王子龙[1] 吴小波[1] 颜伟玉[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所,南昌330045

出　　处：《昆虫学报》2012年第10期1132-1141,共10页Acta Entomologica Sinica

基　　金：高等学校博士学科点专项科研基金(20103603110003);江西省自然科学基金项目(2010GZN0044)

摘　　要：为探究中华蜜蜂Apis cerana cerana dynactin p62基因的表达特性,本研究克隆了中华蜜蜂dynactin p62的基因组DNA序列(GenBank登录号:JX101463)和mRNA序列(GenBank登录号:JX101464);采用荧光定量PCR检测了中华蜜蜂dynactin p62在不同发育时期(3日龄和6日龄幼虫、刚羽化出房蜜蜂)三型蜂中mRNA的表达量。结果表明:该基因基因组DNA序列全长为2403bp,mRNA序列全长为1491bp,编码496个氨基酸残基,预测的蛋白分子量为56.49kD,等电点为8.31。系统发育分析表明中华蜜蜂dynactin p62与西方蜜蜂Apis mellifera dynactin p62聚成一支。该基因在不同发育时期均有表达,在雌性蜜蜂(蜂王和工蜂)中,刚羽化成虫期的表达量显著高于幼虫期(P<0.05),并且同一发育时期相比,工蜂的表达量显著高于蜂王(P<0.05);而该基因在雄蜂中表达量没有明显的规律性。这些结果提示该基因可能与中华蜜蜂级型分化有关。To explore the expression characteristics of dynactin p62 gene in the Chinese honeybee, Apis cerana cerana, the genomic DNA sequence （GenBank accession no. JX101463 ） and mRNA sequence （GenBank accession no. JX101464 ） of dynactin p62 in A. cerana cerana were cloned, and a quantitative analysis of its expression level at different developmental stages of the queen, worker and drone （3 and 6 d-old larvae, and newly eclosed bees） was conducted using real-time PCR. The full- length genomic DNA and mRNA sequences of A. e. cerana dynactin p62 are 2 403 bp and 1 491 bp, respectively, encoding 496 amino acids, and the predicted MW and pI are 56.49 kD and 8. 31, respectively. Phylogenetic analysis indicated that dynactin p62 of both A. c. cerana and A. mellifera gathered in the same clade. The A. c. cerana dynactin p62 transcript was clearly detected at different developmental stages of the queens and workers, and it was expressed significantly higher in newly eclosed adults than in larvae （ P 〈 0.05 ） ; and its expression was higher in workers than in queens （ P 〈 0.05 ）, but with no obvious regularity in drone. These results suggest that dynactin p62 may be involved in caste differentiation in A. c. cerana.

关 键 词：中华蜜蜂 dynactin P62 基因克隆 序列分析 差异表达 

分 类 号：Q966[生物学—昆虫学]