Cry1Fa对Cry1Ac抗性棉铃虫的毒力评价  被引量:3

Evaluation of the toxicity of Cry1Fa to the Cry1Ac-resistant cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

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作  者:魏纪珍[1] 郭予元[1] 高希武[2] 张涛[1,3] 梁革梅[1] 

机构地区:[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]中国农业大学农学与生物技术学院昆虫学系,北京100193 [3]广西大学农学院,南宁530004

出  处:《昆虫学报》2012年第10期1154-1160,共7页Acta Entomologica Sinica

基  金:国家转基因生物新品种培育重大专项课题(2011ZX08011-002)

摘  要:为了防治多种鳞翅目害虫,表达Cry1Fa的转基因玉米和棉花已在美国商业化种植。明确棉铃虫Helicoverpa armigera对Cry1Fa与Cry1Ac的交互抗性及这两种杀虫蛋白之间的协同作用,可以为表达Cry1Fa+Cry1Ac的转双价抗虫棉花的合理应用提供依据。本实验测定了Cry1Fa对棉铃虫敏感品系(96S)及用Cry1Ac筛选的抗性品系(BtR,抗性倍数2194.15倍)的毒力,发现Cry1Fa对敏感棉铃虫的毒力远低于Cry1Ac,LC50值是Cry1Ac的504.80倍;而且抗性品系BtR对Cry1Fa存在19.98倍的交互抗性。Cry1Fa与Cry1Ac混用可以提高Cry1Fa毒杀敏感棉铃虫的效果,尤其是Cry1Fa浓度较低时,加入Cry1Ac,可以显著增加Cry1Fa的毒力;但只有加入较高浓度的Cry1Fa时才能增加Cry1Ac的毒力。由于BtR品系已经对Cry1Ac产生抗性,Cry1Ac对抗性棉铃虫的毒力明显降低;在较高浓度的Cry1Ac中加入Cry1Fa可以显著增加棉铃虫的死亡率(P=0.0015,F=6.88,df=6),但最高死亡率仅为58.33%。D-饱和最优试验的结果证实,Cry1Ac对于敏感棉铃虫的死亡率的影响达到显著水平(t1=13.76﹥t0.05),Cry1Ac与Cry1Fa的交互作用对毒力的影响也达到显著水平(t22=2.42﹥t0.05;t11=6.95﹥t0.05;t12=3.43﹥t0.05)。Cry1Ac和Cry1Fa对抗性棉铃虫死亡率的影响都达到显著水平(t1=3.03﹥t0.05;t2=2.59﹥t0.05),但Cry1Ac是决定抗、感棉铃虫死亡率的关键因素;Cry1Ac与Cry1Fa最佳浓度配比范围都是1.41~2.10μg/cm2;在抗性品系中,Cry1Ac和Cry1Fa的交互作用不显著。所以,尽管Cry1F+Cry1A作物扩大了杀虫谱,但棉铃虫对这两种蛋白存在交互抗性,而且这两种蛋白混用对治理抗Cry1Ac棉铃虫的效果不理想,因此不建议在中国种植表达Cry1F+Cry1A的棉花。Cry1Fa-expressing corn and cotton have been commercially planted in U. S in order to effectively control several Lepidoptera pests. Making clear the cross-resistance of cotton bollworms (Helicoverpa armigera) to Cry1Ac and Cry1Fa and the synergism between the two toxins could provide the theoretical bases for rationally applying Cry1Fa + Cry1Ac cotton. The toxicity of Cry1Fa to the susceptible strain (96S) and the resistant strain (BtR, selected by Cry1Ac, the resistance ratio was 2 194.15-fold) was tested in our study. The results showed that the toxicity of Cry1Fa was lower than Cry1Ac to 96S, the LC50 value of Cry1Fa was 504.80 times as high as that of Cry1Ac, and there was 19.98-fold cross-resistance to Cry1Fa in the Cry1Ac-resistant strain (BtR). Cry1Fa mixed with Cry1Ac could increase the toxicity of Cry1Fa against 96S, and especially when the concentration of Cry1Fa was low, the synergistic effect was significant. However, the toxicity of Cry1Ac could be improved just when being added with high-dose Cry1Fa. Because the BtR strain had resistance to Cry1Ac, the toxicity of Cry1Ac to BtR reduced obviously. Mixing Cry1Fa into high-dose Cry1Ac could increase the mortality of BtR (P =0.0015, F =6.88, df=6), but the highest mortality was only 58.33%. The results of the 2-D-optimum orthogonal design experiment showed that Cry1Ac had significant effect to control the susceptible cotton bollworm (t1 = 13.76 〉 t0.05 ), and the influence of interactive effect between Cry1Ac and Cry1Fa on toxicity was also significant (t22 = 2.42 〉 t0.05 ; t, = 6.95 〉 t0.05 ; t12 = 3.43 〉 t0.05 )- Cry1Ac and Cry1Fa had significant effect against the resistant cotton bollworm (t1 = 3.03 〉 t0.05 ; t2 = 2.59 〉 t0.05 ), but Cry1Ac was the key determinant for the mortality of the susceptible and resistant cotton bollworms. The optimum concentration ranges of Cry1Ac and Cry1Fa were 1.41 - 2. 10 μg/cm2. However, the interactive effect between Cry1Ac and Cry1Fa was not significant in the BtR resis

关 键 词:棉铃虫 CRY1AC Cry1Fa 毒力 交互抗性 协同作用 

分 类 号:Q965.9[生物学—昆虫学]

 

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