复合型抑菌剂在甘蔗开放式组培中的应用研究  

Application of Compound Bacteriostat in the Open Tissue Culture of Sugarcane

作　　者：卢加举[1] 张正学[1] 彭仕荣[1] 李向勇[1] 李志琴[1] 黄先群[2] 

机构地区：[1]贵州省亚热带作物研究所,贵州兴义562400 [2]贵州省生物技术研究所/贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《热带农业科学》2012年第10期68-71,共4页Chinese Journal of Tropical Agriculture

基　　金：贵州省农科院研究生创新基金(No.2011012);贵州省创新能力建设项目(No.20104007)

摘　　要：以"黔糖5号"甘蔗腋芽外植体为材料,将不同浓度的"黔兴1号"抑菌剂加入甘蔗培养基中,确定"黔兴1号"抑菌剂作为甘蔗开放式组织培养抑菌剂在甘蔗诱导及增殖培养阶段的最佳浓度。结果表明,"黔兴1号"抑菌剂浓度在0.5%时可有效抑制培养基的污染,外植体的成活率高达80%,芽增殖系数为3.1。In this paper,＂Qian Tang No 5＂sugarcane was used as the experimental material to determine the optimal antibacterial, induction culture and proliferation concentrations of ＂Qianxing No 1＂ bacteriostat solution in the medium for open tissue culture of sugarcane. The results showed that the explants survive rate and multiplication coefficient in the open tissue culture were 80% and 3.1 respectively at the concentration of 0.5% ＂Qianxing No 1＂ bacteriostat.

关 键 词：黔兴1号抑菌剂 甘蔗 组织培养 

分 类 号：Q944.6[生物学—植物学] S566.1[农业科学—作物学]