克山病尸检患者心肌组织8-羟基脱氧鸟苷酸和硫氧还蛋白还原酶1及谷胱甘肽过氧化物酶1的表达  

Expression of 8-hydroxy-2-deoxy guanosine, thioredoxin reductase 1 and glutathione peroxidase 1 in myocardium of autopsy patients with Keshan disease

作　　者：裴俊瑞[1] 刘明法[1] 刘阳[1] 冯红旗[1] 张智毅[1] 周令望[1] 钟学宽[1] 王铜[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心卫生部病因流行病学重点实验室,150081

出　　处：《中国地方病学杂志》2012年第6期631-634,共4页Chinese Jouranl of Endemiology

基　　金：黑龙江省教育厅科技研究项目（11541209）

摘　　要：目的观察克山病尸检患者心肌组织8-羟基脱氧鸟苷酸（8-OH—dG）、硫氧还蛋白还原酶1（TrxRl）、谷胱甘肽过氧化物酶1（GPxl）的表达，探讨氧化应激反应和硒蛋白水平与克山病发病之间的关系。方法收集8例克山病患者（克山病组，包括4例急型克山病患者、4例慢型克山病患者）和9例非克山病（对照组）心肌尸检样本，采用免疫组化技术检测8-OH—dG、TrxRl和GPxl在心肌组织中的表达。使用Olympus Image—Pro Plus6．0软件对各指标进行定量分析。结果克山病组心肌细胞核8-OH—dG表达阳性率[（68．6±2014）％]显著高于对照组[（2．4±1．5）％，t=8．515，P〈0．05]；而且，急型克山病患者阳性率[（91．74±3．7）％]明显高于慢型克山病患者[（53．2±7．9）％，t=6．409，P〈0．05]。克山病患者心肌组织中抗氧化酶TrxR1和GPxl表达分布与病变灶分布无相关性，且蛋白表达的累积光密度（401340±59865、497590±197082）均低于对照组（2790300±379298、1348400±615840；t值分别为～28．493、-6．016，P均〈0．01）。结论克山病患者心肌组织存在氧化损伤，并且8-OH—dG表达与克山病患者病变程度有关；TrxRl和GPxl可能是与克山病发病关系密切的硒蛋白。Objective In this study, we investigated the relationship between oxidative stress, selenoproteins level and onset of Keshan disease （KD） through detecting the expression of 8-hydroxy-2-deoxy guanosine（8-OH-dG）, thioredoxin reductase 1 （TrxR1） and glutathione peroxidase 1 （GPxl） in myocardial tissue. Methods Myocardium samples of autopsy patients including 8 cases of KD（KD group included 4 acute KD and 4 chronic KD） and 9 cases of non-KD（control group） were immunohistochemieally stained for 8-OH-dG, TrxR1 and GPxl. The staining intensities subsequently quantified by using Olympus Image-Pro Plus 6.0 software. Results The positive rate of 8-OH-dG expression in myocardial nuclei was higher in the case group [ （68.6 ± 20.4）% ] than that of the control group[ （2.4 ± 1.5）%, t = 8.515, P 〈 0.05]. In addition, the positive rate of 8-OH-dG expression in acute KD[（91.7 ± 3.7）%] was significantly higher than that of chronic KD[（53.2 ± 7.9）%, t = 6.409, P〈 0.05]. The distribution of TrxR1 and GPxl was not associated with the distribution of myocardial damage. The expression of these two selenoproteins in KD group （401 340 ± 59 865, 497 590 ± 197 082） were both lower than that of control group（2 790 300 ± 379 298, 1 348 400 ± 615 840; t = - 28.493, -6.016, respectively, all P〈 0.01）. Conclusions Oxidative damage is detected in myocardium tissue of KD, and 8-OH-dG expression is associated with the degree of rfiyocardial damage in KD. Selenoproteins, TrxR1 and GPxl, may be closely related to the pathogenesis of KD.

关 键 词：克山病 8-羟基脱氧鸟苷 谷胱甘肽过氧化酶 硫氧还蛋白还原酶 心肌 人体 

分 类 号：R542.3[医药卫生—心血管疾病]