贵州省首例人间布鲁杆菌病病例的病原学诊断与分析  

Etiologic diagnosis and analysis of the first case of human brucellosis in Guizhou province

作　　者：李世军[1] 王月[1] 王定明[1] 田克诚[1] 刘英[1] 马青[1] 刘昭宾[1] 龚晓俊[1] 唐光鹏[1] 陈贵春[1] 

机构地区：[1]贵州省疾病预防控制中心传染病防治研究所检验科,贵阳550004

出　　处：《中国地方病学杂志》2012年第6期643-645,共3页Chinese Jouranl of Endemiology

基　　金：贵州省高层次人才科研条件特助经费项目（TZJ-2010-100号）;贵州省科学技术基金（黔科合J字[201012191号）

摘　　要：目的病原学诊断与分析贵州省1例布鲁杆菌病疑似病例血液中的菌株，为贵州省首例人间布鲁杆菌病的确诊提供实验依据。方法采用传统方法和分子生物学方法[布鲁杆菌属特异性表面蛋白基因PCR（BCSP31-PCR）和布鲁杆菌特异性PCR（AMOS—PCR）法]对从布鲁杆菌病疑似患者血液分离的可疑布鲁杆菌进行鉴定。结果来自布鲁杆菌疑似患者血液的可疑菌株经传统方法鉴定为羊种生物3型布鲁杆菌；经BCSP31-PCR法鉴定为布鲁杆菌属细菌；经牛（A），羊（M）、绵羊附睾（0）和猪（S）种／型AMOS—PCR法鉴定为羊种布鲁杆菌。结论分离自贵州省布鲁杆菌疑似患者血液的菌株确诊为羊种生物3型布鲁杆菌，为贵州省首例人间布鲁杆菌病病例。Objective To etiologically diagnose and analyze a patient with suspected cases of brucellosis, and to provide a experimental basis for the confirmation of the first case of human brucellosis in Guizhou province. Methods Conventional and molecular techniques[genus specific BruceUa surface protein 31 PCR（BCSP31-PCR） and BruceUa suis species-specific PCR （AMOS-PCR） ] were used to identify suspicious bacteria strains isolated from the suspected patient of brucellosis. Results The results showed that the Brucella suspicious colonies were identified as Brucella melitensis biotype 3 using conventional tests and were further identified as Brucella spp. by genus specific Brucella surface protein 31 PCR （BCSP31-PCR） and classified as Brucella melitensis with Brucella abortus, BruceUa melitensis, Brucella ovis, Brucella suis species-specific PCR（AMOS-PCR）. Conclusions Laboratory diagnostic results show that the bacteria strain isolated from the suspected patient of brucellosis is Brucella melitensis biotype 3. It is the first case of human brucellosis in Guizhou province.

关 键 词：布鲁杆菌病 聚合酶链反应 生物学鉴定法 

分 类 号：R516.7[医药卫生—内科学]