酶联免疫吸附试验捕获法检测牧犬血清中鼠疫IgM抗体  

Detection of plague IgM antibody in the serum of herding dogs by capture enzyme-linked immunosorbent assay

作　　者：王信惠[1] 徐恩洁[2] 雷刚[1] 廖力夫[1] 热娜·吐尔地[1] 涂杰[1] 阿不力米提·买托呼提[1] 李冰[1] 徐秉臣[1] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心鼠疫防治科,乌鲁木齐830002 [2]新疆医科大学第一附属医院检验科

出　　处：《中国地方病学杂志》2012年第6期681-683,共3页Chinese Jouranl of Endemiology

基　　金：十一五国家科技支撑计划（2007BA107A14）;新疆高新技术研究发展计划（200611112）

摘　　要：目的建立酶联免疫吸附试验（ELISA）捕获法，检测牧犬血清中鼠疫IgM抗体。方法用抗犬血清IgM抗体包被反应板，捕获牧犬血清样本中的IgM，并用ELISA夹心法同步检测牧犬血清中鼠疫F1抗体。结果共检测牧犬血清216份，其中ELISA夹心法检出鼠疫F1抗体阳性26份，阳性率为12．03％（26／216），ELISA捕获法检出IgM抗体阳性14份，阳性率为6．48％（14／216），占总阳性的53．85％（14／26）。结论ELISA捕获法检测牧犬鼠疫IgM抗体，可间接推算鼠疫疫源地近期动物鼠疫流行时间和分布，为及时实施防控措施提供参考资料。Objective To establish a capture enzyme-linked immunosorbent assay（ELISA） for detection of plague IgM antibody in herding dogs. Methods ELISA plates were coated with serum IgM antibody against dogs and F1 antibody to plague in the serum of herding dogs was detected by a sandwich ELISA. Results A total of 216 serum samples of herding dogs were tested, 26 were positive for plague F1 antibody and the positive rate was 12.03% （26/216）; 14 were positive for plague IgM antibody and the positive rate was 6.48% （14/216）; IgM positive accounted for 53.8%（14/26） of all positive samples. Conclusions Serum plague IgM antibody of herding dogs can be used to predict the prevalent time and distribution of recent animal plague in plague loci indirectly, and to provide reference information for timely implementation of control measures.

关 键 词：鼠疫 免疫球蛋白M 酶联免疫吸附测定 

分 类 号：R446.6[医药卫生—诊断学]