分次与一次肝部分切除术建立大鼠肝再生模型的对比研究  

Comparison between staging partial hepatectomy and by one time to built liver regeneration model in rats

作　　者：刘国华[1] 戴东[1] 李明意[1] 张谷裕[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524001

出　　处：《中国实验诊断学》2012年第11期2011-2013,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金资助项目(81041099);广东省自然科学基金资助项目(S2011010003750);广东医学院附属医院博士科研基金;广东医学院青年基金;广东医学院附属医院青年基金(2009K04)

摘　　要：目的分别采用分次与一次肝部分切除术方法建立大鼠肝再生模型,通过对比研究以期为研究肝再生规律提供更合理技术平台。方法 SD大鼠随机分为3组:①分次肝部分切除组(A);②一次肝部分切除组(B);③对照组(C)。比较各组切除肝重、残肝重、全肝重与切肝比例,同时比较前两组胆漏、出血、腹腔感染及下腔静脉狭窄并发症发生率,对比分析不同手术方法对肝再生量化的准确性差异及手术建模成功率的影响。结果各组全肝重及肝脏指数其差异统计学上无意义(P>0.05),分次肝部分切除组与对照组比较,切除肝重、残肝重与切肝比例统计学上无意义(P>0.05),而一次肝部分切除组与分次肝部分切除组及对照组三组对比,切除肝重、切肝比例均比后者小(P<0.05)、残肝重则比后者大(P<0.05)。一次切除组胆漏、出血、腹腔感染及下腔静脉狭窄并发症发生率均比分次肝部分切除组高(P<0.05)。结论采用分次肝部分切除术建立大鼠肝再生模型,量化肝脏切除程度的准确性高,并发症发生率低,手术成功率高,是理想的手术方法。Objective By a contrast studies between partial hepatectomy step by step and by one time to built liver regeneration model in rats,we hope to establish a more rational rat model of liver regeneration by partial hepatectomy,so as to provide a basis for investigating the mechanisms of liver regeneration.Methods SD rats were randomized into 3 groups:PH step by step group(group A);②PH by one time group(group B);③control group(group C).we determined whole liver mass、the removed liver mass、the residual liver mass and cut liver proportion.And we comparative analysed the difference of complications such as bile leakage、haemorrhage、celiac infection and the inferior vena cava stricture between group A and group B to find the influence of different surgical procedure on liver regeneration quantification and success rate of surgery.Results The difference of whole liver mass and liver index among three group is statistically insignificant(P>0.05).As compared with group C,the difference of the removed liver mass、the residual liver mass and cut liver proportion isn't significant statistically(P>0.05).And the removed liver mass and cut liver proportion of group B is lighter than group A and group C(P<0.05).But the residual liver mass of group B is lighter than group A and group C(P<0.05).Complications of bile leakage、haemorrhage、celiac infection and the inferior vena cava stricture of group B are higher than group A(P<0.05).Conclusion Because partial hepatectomy step by step has higher accuracy of quantification liver resection、lower complication rate and higher success rate,so we think that would be a ideal operation method to establish rat liver regeneration model.

关 键 词：肝再生 肝切除术 并发症 

分 类 号：R657.3[医药卫生—外科学]