不同抗凝处理对心梗后循环microRNA检测影响的研究  

The effect of different anticoagulants on determination of circulating microRNA in myocardial infarction

作　　者：张洋[1] 聂宇 于金星[1] 丛祥凤[1] 陈曦[1] 

机构地区：[1]中国医学科学院.北京协和医学院阜外心血管病医院检验科,北京100037 [2]中国医学科学院.北京协和医学院阜外心血管病医院心血管转化医学国家重点实验室,北京100037

出　　处：《中国实验诊断学》2012年第11期2038-2041,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(81000773);北京市优秀人才培养资助(2011B009008000003)

摘　　要：目的比较不同抗凝(EDTA抗凝、枸橼酸钠抗凝、不抗凝)所获得的血浆或血清对心梗后循环microRNA(miRNA)检测的影响。方法分别提取不同抗凝剂处理的胸痛6小时内的心梗患者和健康人的血样中的循环miR-NA,利用TaqMan qRT-PCR方法分别检测EDTA抗凝血浆、枸橼酸钠抗凝血浆,以及血清中的miR-1、miR-133a的含量。结果心梗患者血浆和血清中都能有效检测miR-1和miR-133a的浓度。ROC曲线显示,EDTA血浆中的miR-1的AUC为0.885,miR-133a的AUC为0.797,高于血清及枸橼酸钠抗凝血浆中miR-1和miR-133a的AUC。结论 EDTA抗凝的血浆是检测心梗后miR-1和miR-133a含量的理想样本,其中miR-1在诊断心梗方面具有更好的特异性和灵敏性。Objective To analysis the effects of different anticoagulants on clinical test results for miRNA in myocardial infarction.Methods Separate extraction of different anticoagulant treatment within 6 hours of chest pain in patients with myocardial infarction and circulating miRNA in healthy human blood samples,using TaqMan qRT-PCR methods were used to detect EDTA-plasma,sodium citrate plasma,and the separation gel separation of serum miR-1,miR-133a.Results Effectively in plasma of patients with myocardial infarction and serum concentration of the detection of miR-1 and miR-133a.ROC curve displayed that EDTA plasma AUC of miR-1 with 0.885,miR-133a in the AUC of 0.797,which higher than in serum and plasma of sodium citrate anti-coagulation AUC of miR-1 and miR-133a.Conclusion Anticoagulant EDTA plasma is ideal for detection of content of miR-1 and miR-133a after myocardial infarction samples,of which miR-1 in the diagnosis of myocardial infarction with greater specificity and sensitivity.

关 键 词：心肌梗死 循环MICRORNA 抗凝剂 

分 类 号：R542.22[医药卫生—心血管疾病]