大鼠部分肝移植(PLT)的肝叶切除方法及体会  

Methods and experience of hepatolobectomy for partial liver transplantation (PLT) in rats

作　　者：宋康[1] 贺轶锋[1] 周俭[1] 黄晓武[1] 孙健[1] 王晓颖[1] 王征[1] 潘奇[1] 樊嘉[1] 

机构地区：[1]复旦大学附属中山医院肝癌研究所肝外科,上海200032

出　　处：《复旦学报（医学版）》2012年第6期645-648,共4页Fudan University Journal of Medical Sciences

基　　金：复旦大学青年骨干科研启动基金(B-195)~~

摘　　要：目的通过不同的肝叶切除方法,建立30%和60%比例的大鼠部分肝移植(partial livertransplantation,PLT)模型。方法 60%PLT组,在体切除(n=50)或者离体切除(n=50)肝左外叶、尾叶,保留中叶、右叶、右前叶;30%PLT组(n=50),在体切除肝左外叶、尾叶、右叶、右前叶,仅保留中叶。结果各实验组获取的部分供肝比例与临床部分肝移植相似,移植后存活率较高。结论通过在体切除或者离体切除不同的肝叶组合,建立30%和60%比例大鼠PLT模型,能较好地模拟临床部分肝移植过程,为深入进行肝移植的基础研究提供了良好平台。Objective To establish the models of 30% and 60% partial liver transplantation （PLT） in rats by different methods of hepatolobectomy. Methods The 60% PLT group was resected left lobe and caudate lobe in vivo （n = 50） or in vitro （n = 50） ,retained middle lobe,right lobe and right anterior lobe. The 30% PLT group （n = 50） was resected left lobe, caudate lobe, right lobe and right anterior lobe in vivo,retained middle lobe only. Results The proportion of the partial donor liver in each group was similar to the clinical PLT. It had higher survival rate in each group after liver transplantation. Conclusions The models of 30% and 60% PLT in rats can better simulate the process of clinical PLT by different methods of hepatolobectomy in vivo or in vitro. It provides a good platform for in-depth fundamental research on liver transplantation.

关 键 词：动物模型 肝移植 肝叶切除 大鼠 

分 类 号：R657.3[医药卫生—外科学]