雾化吸入肝素对慢性阻塞性肺疾病模型大鼠IL-8、TNF-α水平的影响研究  

Influence of Aerosol Inhalation of Heparin on IL-8 and TNF-α Levels in COPD Model Rats

作　　者：郭莹[1] 柴文戍[1] 潘殿柱[1] 韩彬[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《中国药房》2012年第45期4243-4245,共3页China Pharmacy

摘　　要：目的:研究雾化吸入肝素对慢性阻塞性肺疾病(COPD)模型大鼠IL-8、TNF-α水平的影响。方法:将大鼠随机均分为对照组、模型(生理盐水)组和肝素高、低剂量(12500、6250u·4mL-1)组,每组10只,后3组气管注入脂多糖并熏香烟以建立COPD模型,各组第8天起雾化吸入相应药物,连续3周。放射免疫法测定血清和支气管肺泡灌洗液(BALF)中IL-8和TNF-α的水平,收集血液和BALF进行白细胞和中性粒细胞计数,观察各组大鼠肺组织切片形态学变化。结果:与对照组比较,模型组大鼠肺泡结构紊乱、肺泡壁变薄断裂,模型组、肝素低剂量组血清和BALF中IL-8及TNF-α水平、白细胞总数、中性粒细胞比例均明显升高(P<0.05或P<0.01);与模型组比较,肝素高剂量组肺组织形态学变化明显好转,血清和BALF中IL-8及TNF-α水平、白细胞总数、中性粒细胞比例明显降低(P<0.05),肝素低剂量组上述指标相比均无明显差异。结论:雾化吸入肝素可能通过限制炎症细胞的活化及组织破坏修复、抑制内皮细胞与白细胞的强黏附过程,发挥减轻COPD炎症的作用。OBJECTIVE：To study the influence of aerosol inhalation of heparin on IL-8 and TNF-α levels in chronic obstructive pulmonary disease（COPD）model rats.METHODS：Rats were randomly divided into control group,model group（normal saline）,high-dose and low-dose heparin groups（12 500,6 250 u·4 mL-1）with 10 rats in each group.Latter 3 groups were given lipopolysaccharide and exposured to cigarette to establish COPD model,and aerosol inhalation of relevant medicine were carried out since 8th day in 4 groups for consecutive 3 weeks.Morphology changes of tissue section of lung were observed in each group.Total WBC counts and nertrophil counts in blood and BALF were examined.The levels of IL-8 and TNF-α in serum and BALF were measured by radioimmunoassay.RESULTS：Compared with control group,alveoli disorder of rats was detected and alveolar wall became progressively thinner to break in model group;the levels of IL-8 and TNF-α,total WBC counts and neutrophil counts in serum and BALF in model group and low-dose heparin group were increased significantly（P0.05 or P0.01）.Compared with model group,morphology changes of lung tissue in high-dose heparin group were improved significantly;IL-8 and TNF-α levels,total WBC counts and neutrophil counts in serum and BALF decreased significantly（P0.05）,and the above index of low-dose heparin group had no significant difference.CONCLUSION：Aerosol inhalation of heparin can reduce COPD inflammation by restricting the activation of inflammatory cells and repair of destroyed tissue,and inhibiting adhesion process of endothelial cell with WBC.

关 键 词：慢性阻塞性肺疾病 大鼠 肝素 雾化吸入 

分 类 号：R563.3[医药卫生—呼吸系统] R965[医药卫生—内科学]