荷移分光光度法测定加替沙星片中主药的含量  

Content Determination of Main Component in Gatifloxacin Tablets by Charge Transfer Reaction Spectrophotometry

作　　者：赵桂芝[1] 

机构地区：[1]辽宁医学院,辽宁锦州121000

出　　处：《中国药房》2012年第45期4305-4307,共3页China Pharmacy

摘　　要：目的:建立测定加替沙星片含量的方法。方法:通过加替沙星与茜素络合指示剂间发生的电荷转移反应,采用紫外-可见分光光度法测定其含量,并与该制剂现行标准测定方法(高效液相色谱法)测定结果进行比较。结果:加替沙星与茜素络合指示剂在乙醇与水混合介质中发生荷移反应,产物最大吸收波长为533nm,表观摩尔吸光系数为2.31×103L·mol-1·cm-1,荷移络合物的组成比为1:1,方法重现性试验中RSD为0.98%(n=6);加替沙星检测浓度线性范围为10～90mg·L-1(r=0.9996),平均回收率为99.9%,RSD=1.02%。2种方法测定样品含量结果一致。结论:建立的方法操作简便、快速,灵敏度高,结果准确。OBJECTIVE：To establish the method for the content determination of Gatifloxacin tablets.METHODS：After charge transfer reaction of gatifloxacin and alizarin complexone,the content of Gatifloxacin tablets was determined by Ultraviolet and visible spectrophotometry,which was compared with result of present standard HPLC method.RESULTS：The charge transfer reaction between gatifloxacin and alizarin complexone took place in methyl alcohol-water.The apparent molar absorption coefficient of complex at 533 nm was 2.31×10 3 L·mol-1·cm-1,and the composition ratio of charge transfer complex between gatifloxacin and alizarin complexone was 1：1,RSD in reproducibility test was 0.98%（n=6）.The linear range of gatifloxacin was 10～90 mg·L-1（r=0.999 6）with an average recovery of 99.9%（RSD=1.02%）.Content determination of 2 methods revealed similar results.CONCLUSION：The method is simple,rapid,sensitive and accurate.

关 键 词：加替沙星片剂 茜素络合指示剂 荷移反应 分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学]