我国3个新记录海链藻种类的形态学研究  

MORPHOLOGICAL STUDIES ABOUT THREE NEWLY RECORDED THALASSIOSIRA SPECIES FOR CHINA

作　　者：李扬[1] 赵秋兰[1,2] 吕颂辉[3] 

机构地区：[1]华南师范大学生命科学学院广东省水产健康安全养殖重点实验室广东省高等学校生态与环境科学重点实验室,广州510631 [2]韩山师范学院陶瓷学院,潮州521041 [3]暨南大学生命科学与技术学院赤潮与水环境研究中心,广州510632

出　　处：《水生生物学报》2012年第6期1041-1047,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(30700046);973项目(2010CB428702)资助

摘　　要：利用光镜(LM)和扫描电镜(SEM)技术,对采自我国沿海海域的自然水样,以及分离获得的单克隆培养藻株进行了形态学观察,针对其中的海链藻属Thalassiosira Cleve种类开展了形态分类学的专题研究。报道了3个新记录种类,分别是安达曼海链藻Thalassiosira andamanica Gedde、缢缩海链藻Thalassiosira con-stricta Gaarder和双线海链藻Thalassiosira duostra Pienaar。对每个种类的形态学特征、生活习性和生态分布进行了描述,提供了光镜和电镜照片,并对相似种类的形态学特征进行了比较研究。Thalassiosira Cleve is a very common diatom genus with high species diversity in coastal waters around the world. Up to now, nearly one hundred and fifty taxa belonging to this genus have already been reported. Thalassiosira is the main contributor to nano-phytoplankton assemblage because cell sizes of most species are below 20 μm. Previously, some Thalassiosira species were considered as model organisms of centric diatoms and Bacillariophyta, and many studies concerning genetics, sexual reproduction, feeding pressure, impact on egg producing and hatching of zooplankton, envi ronmental indicator, etc, have been performed. On the other hand, some Thalassiosira species are also important bloom-causing organisms. And the blooms induced by T. curviseriata and T. diporocyclus have already been reported in the Changjiang River Estuary and along Hainan coast respectively. Due to their very small cell sizes, electron microscopy is needed for accurate identification. In order to characterize the species diversity and distribution of the genus Thalassiosira along Chinese coastal waters, water samples were collected from some locations. Thalassiosira specimens within these samples, as well as several clonal strains isolated from natural samples, were studied by light microscopy （LM） and scan- ning electron microscopy （SEM）. All samples were fixed with Lugo＇s solution and were concentrated to a final volume about 2-10 mL by settling overnight several times. The cultivated samples could be observed and analyzed under a light microscope. For observation under scanning electron microscope, sub samples were taken and treated in several steps. Firstly, after dropped into the same volume of concentrated H2SO4, these samples were boiled in the water bath for about 10-15 minutes to remove the organic materials. Secondly, these samples were washed to neutrality with distilled water for several times. Then, these samples were coated with gold and were subsequently observed, identified and photographed under a scanning elect

关 键 词：海链藻 新记录 中国 形态学特征 

分 类 号：Q94[生物学—植物学]