急性高渗胁迫对中华绒螯蟹雄蟹组织中可溶性蛋白质、血蓝蛋白、血糖与肝糖原含量的影响  

EFFECT OF ACUTE SALINITY STRESS ON SOLUBLE PROTEIN,HEMOCYANIN,HAEMOLYMPH GLUCOSE AND HEPATOPANCREAS GLYCOGEN OF ERIOCHEIR SINENSIS

作　　者：王悦如[1] 李二超[1] 陈立侨[1] 王晓丹[1] 张凤英[2] 高露姣[2] 龙丽娜[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]中国水产科学研究院东海水产研究所,上海200090

出　　处：《水生生物学报》2012年第6期1056-1062,共7页Acta Hydrobiologica Sinica

基　　金：公益性行业(农业)科研专项(201003020;201203065);"十二五"国家科技支撑计划课题(2012BAD25B00);国家自然科学基金项目(31172422;31001098);上海市中华绒螯蟹产业技术体系建设;上海市科技兴农推广项目(沪农科推字2009-2-1);上海市科委重点项目(10JC1404100);上海市自然科学基金(09ZR1409800);高等学校博士点专项基金(20100076120006);农业部海洋与河口渔业资源及生态重点开放实验室基金部分资助

摘　　要：实验以淡水(0.3‰)处理组为对照,研究了急性高渗胁迫(盐度16‰和30‰)对中华绒螯蟹(Eriocheirsinensis)雄蟹血淋巴和肝胰腺中可溶性蛋白质、血蓝蛋白、血糖及肝糖原含量的影响。结果显示,与对照组相比,进入16‰及30‰盐度后,蟹的血淋巴中可溶性蛋白质含量从6—48h显著降低(P<0.05),至72h时显著增加(P<0.05),而机体肝胰腺的可溶性蛋白质含量从12h开始至96h显著降低(P<0.05);血蓝蛋白含量在前24h显著降低(P<0.05),48h开始显著升高(P<0.05);肝糖原含量在6—96h与对照组相比有显著的降低(P<0.05);16‰盐度组蟹的血糖含量在24—48h时显著下降(P<0.05),到72h开始逐渐恢复(P>0.05),而30‰盐度组其血糖含量早在6—12h时就有显著的下降(P<0.05),到24h则开始逐渐升高。研究表明,在急性盐度胁迫下,糖类和蛋白质对中华绒螯蟹渗透压调节起重要的作用,其优先利用糖类的分解获得所需的能量,其中盐度越高,机体内葡萄糖的消耗越快,血糖水平的恢复也相应较快。对蛋白质的利用稍次之,机体可利用蛋白质的分解获得其渗透压调节的效应物游离氨基酸以维持渗透压平衡。Most of the decapod species of crustacean have comprehensive capacity of osmoregulation, and they can adapt to relative wide ambient salinity range. It is important to understand the underlying mechanisms of their comprehensive adaptability to salinity change because this knowledge is helpful for inland culturing of marine species. As a euryhaline crustacean, the Chinese mitten crab, Eriocheir sinensis, has been well studied in many aspects related to their physiological responses to salinity change, and many useful literature findings have been accumulated. Therefore, E. sinensis can be considered as an animal model for studying osmoregulation mechanism of the decapod species. However, the roles of nu trients in osmoregulation of E. sinensis are still limited. In this paper, male E. sinensis in freshwater （0.3%0） were trans ferred directly into waters at salinity values of 16‰ and 30‰, respectively, and the crabs kept at fresh water served as the control. Each treatment has three replicates. The soluble protein in haemolymph and hepatopancreas, hemocyanin, haemo lymph glucose and hepatopancreas glycogen at Oh, 6h, 12h, 24h, 48h, 72h and 96h ofE. sinensis at different salinities were determined. Soluble protein of haemolymph and hepatopancreas were measured with a Folin reagent based on a standard curve using bovine serum albumin as the standard protein. Hemocyanin was assayed using an ultraviolet absorption meth ods at 335 nm and calculated according to Nickerson E1%/1cm =2.83. Haemolymph glucose was assayed using glucose oxi dase-peroxide enzyme （GOD-POD） method. Hepatopancreas glycogen was measured using the anthrone colorimetry method. The results showed that the crabs at salinity 16‰ and 30‰ had significantly lower soluble protein content of hepatopancreas than those at fresh water （P〈0.05） when crabs had encountered salt water for 12h-96h, and the soluble protein content of haemolymph decreased significantly from 6h to 48h, and then increased at 72h. The hemocyanin content showed a sig

关 键 词：盐度 中华绒螯蟹 可溶性蛋白 血蓝蛋白 肝糖原 血糖 

分 类 号：Q142[生物学—生态学]