我国沿海闭合突海链藻的形态学研究  

MORPHOLOGICAL STUDIES OF OCCLUDED PROCESSES IN THALASSIOSIRA SPECIES FROM CHINA

作　　者：李扬[1] 赵秋兰[1,2] 吕颂辉[3] 

机构地区：[1]华南师范大学生命科学学院广东省水产健康安全养殖重点实验室广东省高等学校生态与环境科学重点实验室,广州510631 [2]韩山师范学院陶瓷学院,潮州521041 [3]暨南大学生命科学与技术学院赤潮与水环境研究中心,广州510632

出　　处：《水生生物学报》2012年第6期1097-1105,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(30700046);973项目(2010CB428702)资助

摘　　要：闭合突是一类特殊的突起,仅报道于少数海链藻种类,其形态学特征和排列方式是重要的分类学依据。我国关于闭合突海链藻的报道较少。研究利用扫描电镜(SEM)技术,对采自我国沿海海域的自然水样,以及分离获得的单克隆培养藻株进行了形态学观察,针对其中的海链藻属Thalassiosira Cleve种类开展了形态分类学的专题研究。报道了5个具有闭合突的海链藻种类,分别是西达礁海链藻T.cedarkeyensis Prasad、偏心海链藻T.eccentrica(Ehrenberg)Cleve、伦德海链藻T.lundiana Fryxell、细孔海链藻T.punctigera(Castracane)Hasle和管状海链藻T.tubifera Fryxell,其中有3个为我国新记录种类:西达礁海链藻、细孔海链藻和管状海链藻。对每个种类的形态学特征和生境进行了描述,提供了扫描电镜照片,对相似种类的形态学特征进行了比较研究,分析了突起特征的变化类型及其分类学价值。Thalassiosira Cleve is a common diatom genus with high species diversity in coastal waters around the world Till now, nearly one hundred and fifty taxa belonging to this genus have been reported. Thalassiosira is the main con tributor to nano phytoplankton assemblage because cell sizes of most species are below 20 μm. Previously, some Tha lassiosira species were considered as good model organisms of centric diatoms and Baeillariophyta. Many studies con cerning genetics, sexual reproduction, feeding pressure, impact on egg producing, hatching of zooplankton, and envi- ronmental indicator have been performed. On the other hand, some Thalassiosira species are also important bloom-causing organisms. Algal blooms induced by T. curviseriata and T. diporocyclus have been reported in the Changjiang River Estuary and coast of Hainan Province, respectively. Due to very small cell sizes, electron microscopy （EM） is needed for accurate identi6cation. In order to characterize species diversity and distribution of the genus Tha lassiosira along Chinese coastal waters, water samples were collected from different locations. Thalassiosira specimens within these samples as well as several clonal strains isolated from natural samples were studied using scanning electron microscopy. All samples were fixed with Lugo＇s solution and were concentrated to a final volume about 2-10 mL by settling overnight for several times. For observation under scanning electron microscope, sub-samples were taken and treated in several steps. Firstly, after the same volume of concentrated H2SO4 was added, these samples were boiled in the water bath for about 10-15 minutes to remove organic materials. Secondly, these samples were rinsed with distilled water several times to rid the acid. Then, these samples were coated by gold and thus could be observed, identified, and photographed under scanning electron microscope. The morphology and pattern of processes, including three types, fultoportula, rimoportula and occluded process, are key taxonomic c

关 键 词：海链藻 闭合突 形态学 中国沿海 

分 类 号：Q94[生物学—植物学]