六味地黄汤对BALB/c小鼠海马在体LTP的影响  

The effect of Liuwei Dihuang decoction on long-term potentiation in BALB/c mice in vivo

作　　者：黄晏[1] 胡増峣[1] 刘港[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中药药理与临床》2012年第5期14-17,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年科学基金项目(81202505);"重大新药创制"科技重大专项(2012ZX09J12201-002);全军医学科技"十二五"科研项目(BWS11J052)

摘　　要：目的:考察六味地黄汤对BALB/c小鼠海马在体LTP的影响。方法:六味地黄汤单次或连续灌胃7 d后,考察其对正常BALB/c小鼠及皮质酮致LTP损伤模型动物海马在体LTP的影响。结果:皮下注射皮质酮10～50 mg/kg可剂量依赖性的抑制PS增幅;六味地黄汤单次或连续灌胃7 d对正常BALB/c小鼠PS没有明显影响,但可明显增加皮质酮致LTP损伤模型动物的PS增幅。结论:六味地黄汤对皮质酮致LTP损伤具有明显的改善作用,提示改善神经突触可塑性是六味地黄汤发挥益智作用的机制之一。Objective：Observe the effect of Liuwei Dihuang decoction（LW） on long-term potentiation（LTP） in BALB/c mice in vivo.Methods：BALB/c mice were used in this study,in vivo recording was applied for LTP experiment.Results：Single-time and 7 days oral administration of LW had no effect on LTP in intact BALB/c mice.Corticosterone was administrated by subcutaneous injection 1.5 hours before LTP experiment,and the enhancement of popular spike after tetanic stimulation was significantly inhibited.Single-time and 7 days oral administration of LW increased the enhancement of popular spike after tetanic stimulation in corticosterone treated animals.Conclusion：LW had a protective effect against corticosterone＇s inhibitory effect on LTP,improving LTP contributed to the enhancing effect of LW on learning and memory.

关 键 词：六味地黄汤 皮质酮 长时程增强 

分 类 号：R285[医药卫生—中药学]