β-细辛醚,丁香酚和远志皂苷对Aβ25-35神经细胞毒性的保护作用  

Protective effects of β-asarone,eugenol and tenuigenin on Aβ25-35-induced neurotoxicity in cultured rat hippocampal neurons

作　　者：姜静[1] 邓扬鸥[2] 杨丽[1] 

机构地区：[1]辽宁中医药大学基础医学院药理教研室,沈阳110847 [2]中国医科大学第一临床学院老年病干诊科,沈阳110001

出　　处：《中药药理与临床》2012年第5期25-28,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁省自然科学基金;项目编号(201102151)

摘　　要：目的:优选β-细辛醚,丁香酚和远志皂苷对Aβ25-35诱导的海马神经细胞的保护作用,确定三者最佳配比。方法:采用体外细胞培养的方法,通过NF染色鉴定,建立大鼠海马神经细胞Aβ25-35损伤模型。利用正交实验设计,测定细胞存活率(MTT法)、乳酸脱氢酶(LDH)活性,确定最佳三种药物最佳配比;通过观察细胞形态,利用Hoechst33258染色检测细胞凋亡情况。结果:L9(34)正交设计实验结果表明β-细辛醚(20,40,80μmol/L),丁香酚(2,4,8μmol/L),远志皂苷(10,20,40μmol/L)合用的最佳优化配比剂量分别为40μmol/L,2μmol/L,20μmol/L,可显著降低Aβ25-35(1μmol/L)诱导的神经细胞凋亡率(P<0.01)和LDH活性,提高细胞相对生存率。结论:三种药物联用可有效保护海马神经细胞免受β淀粉样蛋白的毒性损伤。Objective：To detect the synergic protection and determine the best combination of β-asarone,eugenol and tenuigenin on Aβ25-35-induced neurotoxicity in cultured rat hippocampal neurons.Methods：Primary neuronal culture from embryonic rat hippocampi was established and confirmed by neurofilament staining.Neurotoxicity was induced by amyloid beta 25-35（Aβ25-35）.Using orthogonal experimental design,the best combination and synergic protection of β-asarone,eugenol and tenuigenin from Aβ25-35 neurotoxicity was determined through MTT coloriemtry and LDH activity.Apoptotic nuclei were detected by Hoechst 33258 staining viewed under fluorescence microscope.Results：The best combination of β-asarone,eugenol and tenuigenin was 40,2 and 20 μmol/L,which can significantly increase the cell viability and suppress the LDH leakage and apoptosis in cultured neurons（P0.01）.Conclusion：β-asarone,eugenol and tenuigenin can protect the cultured hippocampal cells from Aβ25-35-induced neurotoxicity in synergy.

关 键 词：Β-细辛醚 丁香酚 远志皂苷 AΒ25-35 海马神经细胞 

分 类 号：R285[医药卫生—中药学]