苦木提取物对原发性高血压大鼠的降压作用研究  

Studies of Picrasma quassiodes extracts on the antihypertensive effect in spontaneously hypertensive rats

作　　者：赵文娜[1] 苏琪[1] 何姣[1] 赵丽娜[1] 谢人明[1] 孙文基[1] 

机构地区：[1]西北大学陕西省生物医药重点实验室,西安710069

出　　处：《中药药理与临床》2012年第5期108-111,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：西北大学研究生创新基金(10YZZ34)

摘　　要：目的:探讨苦木提取物(PQB)对原发性高血压大鼠(SHR)的降压作用及其降压机制。方法:40只雄性SHR被随机分为5组,高血压对照组,卡托普利组,PQB高剂量组,PQB中剂量组,PQB低剂量组,每组8只。每日给予各组大鼠相应药物灌胃,高血压对照组给予等量蒸馏水。6周末,自大鼠颈动脉取血,用于测定血清一氧化氮(NO)及超氧化物歧化酶(SOD)含量,同时取胸主动脉组织2cm左右用中性甲醛固定,用免于免疫组织化学法测定内皮一氧化氮合酶(eNOS)表达。结果:与高血压对照组相比,PQB中剂量组(100 mg/kg/d)和高剂量组(200 mg/kg/d)NO及SOD及含量明显升高(分别为P<0.05和P<0.01);而卡托普利组(12.5 mg/kg/d)和小剂量组(50 mg/kg/d)效果不显著(P>0.05)。胸主动脉eNOS蛋白表达逐步增强。结论:PQB可显著升高SHR大鼠血清NO和SOD水平,使eNOS蛋白表达增强,推测其降压机制主要是通过影响胸主动脉eNOS蛋白表达,增加血管舒张因子NO的含量,从而使血管扩张,血压下降。Objective：To explore the antihypertensive effect of extracts from PQB on SHR and its mechanism.Methods：40 male SHR were randomly divided into five groups,SHR control group;captopril group;PQB high dose group;PQB middle dose group and PQB low dose group,each group had eight rats.Given rats corresponding drug orally,the hypertension the control group with distilled water.After 6 weeks treatment,blood was collected from the rat carotid artery for determination of serum nitric oxide（NO） and superoxide dismutase（SOD） activity,at the same time about 2cm of the thoracic aorta was embedded in paraf？n for immunohistochemical analysis of eNOS expression.Results：Compared with the SHR control group,the NO and SOD of PQB administration in middle dose group（100 mg/kg/d） and high dose group（200 mg/kg/d） increased（P0.05 and P0.01）;however,the low dose group（50 mg/kg/d） and captopril group（12.5 mg/kg/d） had no significant effect.The expression of eNOS gradually enhanced.Conclusion：PQB could significantly elevated serum NO and SOD levels of SHR,increased the expression of eNOS,the results suggested that the antihypertensive mechanism may be by increasing expression of important proteins in NO pathway namely eNOS and improving NO availability which infers relaxation of the vessel wall to control blood pressure.

关 键 词：苦木 原发性高血压 一氧化氮 超氧化物歧化酶 蛋白表达 

分 类 号：R285[医药卫生—中药学]