溃结灵含药血清对Caco-2炎症细胞模型泛素系统E1、UBC5和E3RSIB蛋白表达的作用  

The effect of serum containing Kuijieling decotion on protein expression of E1、UBC5、E3RSI B in ubiquitin system of Caco-2 cell model of inflammation

作　　者：柴玉娜[1] 郭珍[1] 杜群[1] 李燕舞[1] 王汝俊[1] 巫燕莉[1] 

机构地区：[1]广州市机场路12号广州中医药大学脾胃研究所,广州510405

出　　处：《中药药理与临床》2012年第5期137-140,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(编号:30873421)

摘　　要：目的:观察溃结灵含药血清对Caco-2炎症细胞模型泛素系统的E1、UBC5、E3RSI B蛋白表达的作用,进一步探讨中药复方溃结灵治疗溃疡性结肠炎的作用机理。方法:将生长融合至70-80%左右的Caco-2细胞分成六个组:正常组、模型组、阳性药组(SASP)、溃结灵高、中、低剂量组;加入对应药物孵育,除正常组外,其余各组加入IL-1β刺激细胞建立炎症细胞模型,收集细胞标本。采用Elisa方法检测Caco-2细胞E1、UBC5和E3RSI B蛋白含量。结果:IL-1β刺激的Caco-2炎症细胞模型中,模型组细胞E1蛋白表达明显高于正常组(P﹤0.05),溃结灵中(10%含药血清)、低剂量组(5%含药血清)E1蛋白表达量低于模型组(P﹤0.05);模型组细胞UBC5蛋白表达高于正常组,但无统计学意义(P﹥0.05),溃结灵高(20%含药血清)、中剂量组(10%含药血清)UBC5蛋白表达量低于模型组(P﹤0.01,P﹤0.05);模型组细胞E3RSI B蛋白表达明显高于正常组(P﹤0.05),溃结灵高(20%含药血清)、中剂量组(10%含药血清)、低剂量组(5%含药血清)E3RSI B蛋白表达量低于模型组(P﹤0.01,P﹤0.05)。结论:溃结灵含药血清对Caco-2炎症细胞模型的E1、UBC5、E3RSI B表达均有下调作用,其抗UC作用的机理可能为抑制泛素蛋白酶体系统来抑制I B的泛素化降解,进而抑制NF-B的活化,减轻溃疡性结肠炎的炎症反应。Objective：To observe the effect of serum containing Kuijieling decotion（KD） on protein expression of E1、UBC5、E3RSI B in ubiquitin system of Caco-2 cell model of inflammation and to further explore the mechanism of chinese herbal compound Kuijieling in treatment of ulcerative colitis.Methods：Caco-2 cell in the growth of 70-80% density were divided into six groups：normal group,model group,positive drug group（SASP）,Kuijieling high dose group,middle dose group,low dose group,then were given corresponding drugs and were stimulated by IL-1βfor the establishment of cell model of inflammation,cells are collected.The E1,UBC5 and E3RSIkB protein content of Caco-2 cells were detected by enzyme-linked immunoabsorbent assay（ELISA） methods.Results：The expression of E1 protein in model group was significantly higher in Caco-2 cell model of inflammation induced by Interleukin-1βthan that in normal controlgroup（P0.05）,the protein expres-sion of E1 in medium-dosage group（10% serum containing KD） and low-dosage group（5% serum containing KD） was lower than that in model group（P0.05）;The protein expression of UBC5 in model group was higher than that in normal group（P0.05）,but it is not statistically significant（P0.05）;the protein expression of UBC5 in high-dosage group（20% serum containing KD） and medium-dosage group（10% serum containing KD） were significantly lower than that in model group（P0.01,P0.05）;the protein expression of E3RSI B in model group was significantly higher than that in normal group（P0.05））;the protein expression of E3RSIkB in three groups（20%,10%,5% serum containing KD） were significantly lower than that in model group（P0.01,P0.05）.Conclusion：KD contained serum have a lowering effect on protein expression of E1、UBC5、E3RSI B in Caco-2 cell model of inflammation,KD may affect the treatment of ulcerative colitis through inhibiting the ubiquitin-proteasome system to prevent the IkBa degradation of ubiquitination,and then inhibit the acti

关 键 词：溃结灵 溃疡性结肠炎 CACO-2细胞 白介素-1Β E1 UBC5 E3RSIB 

分 类 号：R285[医药卫生—中药学]