益阴通络胶囊对局灶性脑缺血大鼠MDA,LDH和VEGF的影响  

Influence of MDA,LDH,VEGF of on focal cerebralischemia in rats of Yiyintongluo Capsule

作　　者：白黎明[1] 杨秀清[1] 张晓双[2] 白璐[2] 

机构地区：[1]陕西中医学院附属医院,咸阳712000 [2]陕西中医学院,咸阳712046

出　　处：《中药药理与临床》2012年第5期164-166,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:考察益阴通络胶囊对局灶性脑缺血模型大鼠血清丙二醛(MDA);乳酸脱氢酶(LDH)及脑组织血管内皮生长因子(VEGF)的影响。方法:采用大脑中动脉阻塞法制备大鼠局灶性脑缺血模型,将造模成功后大鼠按神经学评分随机分为假手术组、模型对照组、阳性对照组、益阴通络胶囊大、中、小剂量组六组,给药两周后进行神经功能学评分、测定血清SOD和MDA含量和免疫组织化学法测定脑组织中VEGF。结果:益阴通络胶囊大剂量组(10.8g/kg)和中剂量组(5.4g/kg)可降低局灶性脑缺血模型大鼠神经行为学评分(P<0.01);大剂量组(10.8g/kg)、中剂量组(5.4 g/kg)和小剂量组(2.7g/kg)可降低血清MDA和LDH含量(P<0.05,P<0.01);大剂量组(10.8g/kg)、中剂量组(5.4g/kg)和小剂量组(2.7g/kg)可增加脑组织中VEGF含量(P<0.05)。结论:益阴通络胶囊对大鼠局灶性脑缺血有保护作用,其作用机制可能与抗自由基、促进新生血管形成有关。Objective：To study the influence of malondialdehyde（MDA）,lactate dehydrogenase（LDH）content of serum,brain vascular endothelial growth factor（VEGF） of on focal cerebral ischemia model in rats of YiyinTongluo capsule.Method：The local cerebral ischemia in rat was made by middle cerebral artery occlusion,Successful model rats were randomly divided into sham operation group,model control group,YiyinTongluo capsule large,medium and small dose group and positive group of six groups according to neurological score.After treatment for two weeks,Measured neurological score,the contents of MDA,LDH content of serum and VEGF by immune histochemical method.Results：high-dose group（10.8g/kg）and middle dose group（5.4g/kg）of YiyinTongluo capsule can decrease neural behavioral score（P0.01）;high-dose group（10.8g/kg）,middle dose group（5.4g/kg）and small dose group（2.7g/kg）can decrease MDA and LDH content of serum;high-dose group（10.8g/kg）,middle dose group（5.4g/kg）and small dose group（2.7g/kg）can increase VEGF content in brain（P0.05）.Conclusion：YiyinTongluo capsule has protective effect on focal cerebral ischemia in rats,and its mechanism may be related to free radical,promote the formation of new blood vessels.

关 键 词：益阴通络胶囊 局灶性脑缺血 丙二醛 乳酸脱氢酶 血管内皮生长因子 

分 类 号：R285[医药卫生—中药学]