RVVC白念珠菌氟康唑敏感株和耐药株对外周血DC_s分化成熟的影响  

Research on the Effects of the Fluconazole resistance and Fluconazole susceptible Candida Albicans Strains in RVVC on the Shapes and the Surface Molecules of the Human Peripheral Blood Monocyte-Derived DC_s

作　　者：宣晓梅[1] 刘静[1] 沈丽[1] 刘瑞琴[1] 李艳佳[1] 刘丽娟[1] 李英涛[1] 于亮[1] 

机构地区：[1]河北医科大学第一医院皮肤科,河北石家庄050031

出　　处：《中国皮肤性病学杂志》2012年第12期1075-1079,1082,共6页The Chinese Journal of Dermatovenereology

基　　金：河北省卫生厅重点科技研究计划(20090336)

摘　　要：目的 探讨复发性外阴阴道念珠菌病(RVVC)患者阴道白念珠菌对氟康唑敏感情况及其对人外周血单核细胞源树突状细胞(DCs)形态及表面分子的影响。方法 科玛嘉念珠菌显色培养基及芽管试验分离、鉴定白念珠菌;MIC法判断敏感菌及耐药菌;体外培养正常人外周血单核细胞源DCs,实验组以不同剂量氟康唑敏感菌悬液、耐药菌悬液刺激,对照组以同等剂量标准菌悬液刺激后,倒置显微镜观察细胞形态变化,流式细胞仪检测细胞表面分子的表达。结果 RVVC患者阴道分泌物共分离出白念珠菌108株,其中对氟康唑敏感67株(62.04%),耐药41株(37.96%);各实验组与对照组DCs细胞形态无明显差别。DCs表面分子CD80,CD86表达量均与白念珠菌悬液剂量呈正相关;加入标准菌株悬液的对照组DCs表面分子CD80,CD86表达明显高于同等剂量加入敏感菌悬液和耐药菌悬液的实验组,且加入敏感菌悬液的实验1组DCs表面分子CD80,CD86表达明显高于同等剂量加入耐药菌悬液的实验2组。结论 在一定剂量范围内,白念珠菌可促进DCs表面分子CD80,CD86表达,使DCs进一步成熟,氟康唑敏感菌株优于耐药菌株,但两者均不及标准菌株。Objective To investigate the effects of the fluconazole resistance and fiuconazole susceptible candida albi- cans strains in recurrent vulvovaginal eandidiasis （RVVC） on the Shapes and the Surface Molecules of the Human Peripheral Blood Monocyte-Derived dendritic cells （DCs）. Methods Candida albicans in RVVC were separated and were judged by the CHROMagar Monilia colouration nutritive medium and the germ-tube- formming tests, and then were judged the fluconazole-resistance or fluconazole-usceptible strains by MIC tests. The dendritic cells were obtained by culture in vitro. Then after they were stimulated by the different doses of candida albicans including the fluconazole-esistance and fluconazole-usceptible strains of experimen- tal groups and the ATCC10231 strains of control groups,we observed the shapes of the cells by the inverted microscope and analyzed the expressions of CDS0 and CD86 by FACS. Results There were 67 （62.04%） fluconazole-usceptible strains and 41 （37.96%） flueonazole-esistance strains of 108 candida albicans. The levels of CD80 and CD86 expressions of the control groups were much higher than those in the same dose of experimental groups （P 〈 0.05 ）. While the expressive levels of CD80 and CD86 of fluconazole-useeptible strains in experimental groups 1 were much higher than that of fluconazole-esistance strains in experimental groups 2 （P 〈 0.05 ）. Furthermore the levels correlated to the doses of candida albicans directly （ P 〈 0.05 ）. But there were no changes of the shapes of the cells in the two groups. Conclusion In the definite doses, candida albicans can enhance the expressions of CD80 and CD86, and then enhance the maturation of the dendritic cells. The ATCC10231 strains play best, while the fluconazole-usceptible strains play better than the fluconazole-resistance strains in this point.

关 键 词：复发性外阴阴道念珠菌病 白念珠菌 氟康唑 树突状细胞 

分 类 号：R711.31[医药卫生—妇产科学]