多主棒孢菌调控致病性相关基因CCK1的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Gene CCK1 Related to Pathogenicity of Corynespora cassiicola on Hevea Rubber

作　　者：刘晓妹[1,2] 蒲金基[3] 张欣 漆艳香[3] 谢艺贤[3] 张贺[3] 郑服丛[1] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海口570228 [3]中国热带农业科学院环境与植物保护研究所,儋州571737

出　　处：《生物技术通报》2012年第10期168-172,共5页Biotechnology Bulletin

基　　金：科技部科技支撑计划项目(2007BAD48B04);国家橡胶产业技术体系(CARS-34-GW8)

摘　　要：采用同源克隆、SEFA-PCR和RT-PCR技术克隆获得了多主棒孢菌(Corynespora cassiicola)CCK1基因的DNA和cDNA。生物信息学分析表明,该基因DNA和cDNA全长分别为2 710 bp和1 065 bp,编码区含4个内含子(长53、54、50和56bp),推测编码354个氨基酸,其分子量约40.98 kD,等电点(pI)6.43。从GenBank搜索相似蛋白和构建进化树发现,CCK1与多个丝状真菌调控致病性相关的PMK1类MAPK蛋白聚为一类,且含1个参与双重磷酸化作用的MAPK蛋白激活域TEY和1个Ser/Thr蛋白激酶保守结构域。推测CCK1基因可能参与调控C.cassiicola菌丝生长、产孢和致病性等。A new gene CCK1 of unknown function was cloned from Corynespora cassiicola（Bert.Curt.）Wei on Hevea rubber by the method of homologous cloning,SEFA-PCR and RT-PCR.The bioinformatics analysis results were as follows：The complete DNA and cDNA sequence of CCK1 gene were respectively 2 710 bp and 1 065 bp in size,including four introns with 56,54,50,and 56 bp,respectively,encoding a putative protein of 354-amino acid with a molecular weight 40.98 kD and isoelectric point of 6.43.After searching for proteins similar to CCK1 in the GenBank database and conducting a phylogenetic tree analysis,putative protein of CCK1 was found to be greatly similarity to some other fungal PMK1-type MAP kinases related to regulating pathogenicity.Furthermore,a TEY of the MAP domain-participating dual phosphorylation and Ser/Thr kinase-conserved domain were included.The protein kinase CCK1 may involve in mycelia growth,conidiation,and pathogenicity of C.cassiicola.

关 键 词：多主棒孢菌 蛋白激酶 克隆 生物学信息 

分 类 号：S432.4[农业科学—植物病理学]