一株南海北部深海沉积环境真菌00457的鉴定及其活性研究  

Identification and Activities of Fungal Strain 00457 Isolated from the Deep-sea Sediment of Northern South China Sea

作　　者：黄智[1,2] 王发左[1] 田新朋[1] 李洁[1] 张偲[1] 

机构地区：[1]中国科学院南海海洋研究所中国科学院海洋生物资源可持续利用重点实验室,广州510301 [2]中国科学院研究生院,北京100049

出　　处：《生物技术通报》2012年第10期199-204,共6页Biotechnology Bulletin

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB833800);国家自然科学青年基金项目(40906076;40906075);中国科学院知识创新工程重要方向项目(KSCX2-EW-G-12);财政部战略生物资源科技支撑运行专项项目(KSCX2-YW-Z-1018)

摘　　要：从南海深海沉积环境样品中分离到一株编号为00457的真菌,基于形态学特征、ITS和5.8S rDNA序列比对分析,鉴定该菌株为白黄笋顶孢霉(Acrostalagmus luteoalbus)。活性研究表明,其代谢产物粗浸膏的卤虫致死活性明显,并具有一定强度的抑菌和DPPH自由基清除活性。此外,研究还发现代谢产物粗浸膏在25-100℃持续加热不超过30 min时,卤虫致死活性成分稳定,但在100℃持续加热超过60 min后卤虫致死活性成分显著减少,而一定强度的紫外光照射90 min内则无显著影响。Based on the morphological characteristics and ITS-5.8S rDNA gene sequences analysis,a strain named 00457 isolated from deep-sea sediment of northern South China Sea,was identified as a member of Acrostalagmus luteoalbus.Activity test for the crude extract of fungal strain 00457 showed that it had obvious brine shrimp lethality,as well as potential antibacterial activity and DPPH radical scavenging activity.Moreover,the brine shrimp lethal activity was stable under the conditions of heating at 25-100℃ for 30 min,while decreased after heating at 100℃ for more than 60 min.UV radiation for 90 min did not affect the brine shrimp lethality of crude extract.

关 键 词：真菌 深海沉积环境 鉴定 抗菌活性 抗氧化活性 卤虫致死活性 

分 类 号：Q93[生物学—微生物学]