亚砷酸钠单次染毒大鼠后唾液及血液总砷含量变化比较  

Comparison of the total arsenic concentration between saliva and blood after oral administration of sodium arsenite to rats

作　　者：王大朋[1] 范丽丽[2] 张利明[3] 李建[3] 刘建[1] 金洹宇[1] 刘星[1] 安艳[1] 

机构地区：[1]苏州大学医学部公共卫生学院卫生毒理学教研室,苏州215123 [2]苏州大学医学部公共卫生学院营养与食品卫生教研室 [3]苏州市疾病预防控制中心检验科

出　　处：《卫生研究》2012年第6期947-950,共4页Journal of Hygiene Research

基　　金：日本文部科学省日本学术振兴会研究辅助金资助项目(No.20390173);苏州大学国家自然科学基金预研项目(No.Q3126982)

摘　　要：目的比较亚砷酸钠单次染毒后大鼠血液及唾液中总砷含量随时间变化情况。方法健康清洁级SD大鼠21只,适应性饲养一周后一次性灌服亚砷酸钠20mg/kg。于给药前(0 h)和给药后1～2、4～5、7～8、11～12、17～18和23～24 h时间段分别收集血液和唾液,利用原子荧光分光光度计(AFS-230)检测血砷含量,电感耦合-等离子体质谱(ICP-MS)测定唾液砷含量。结果大鼠摄入亚砷酸钠后,其血液中砷含量急剧上升,在1～2小时便达到峰值,随后下降,但在第7～8小时又出现第二个峰值。唾液中砷含量上升趋势较血砷缓慢,在第7～8小时达到峰值,随后逐渐下降。唾液砷与血砷随时间变化趋势基本一致,且两者之间存在明显的正相关性,r=0.678(P<0.01)。结论砷在唾液中的代谢及排泄速率与血液相比较为缓慢,但总体趋势基本一致,表明唾液砷也能够反映机体砷暴露水平。Objective To compare the total arsenic concentration between blood and saliva after oral administration of sodium arsenite to SD rats.Methods A single oral gavage dose of sodium arsenite（20mg/kg） was administrated to 21 adult male Sprague-Dawley rats.Then collected blood and saliva samples at 0,1-2,4-5,7-8,11-12,17-18,23-24 hour for total arsenic detection.The blood samples were detected for total arsenic concentration by Atomic Fluorescence Spectrometry（AFS-230） and the salivary arsenic were detected by inductively coupled plasma mass spectrometry（ICP-MS）.Results After intake of 20mg/kg BW sodium arsenite,the total arsenic concentration in blood of SD rats was increased rapidly,and reached the peak value at the 1-2 hour,then descended gradually.However,there was a second peak value at the 7-8 hour.The upward trend of salivary arsenic was more slowly than blood arsenic,and reached the peak value at the 7-8 hour,then descended gradually.The variation tendency of salivary arsenic and blood arsenic with time were basically the same.Besides,there was an obvious positive association between them,the correlation coefficient was 0.678,P0.01.Conclusion The excretion of arsenic in saliva was slower than that of blood samples after administrated a single oral gavage dose of sodium arsenite（20mg/kg） to SD rate,but the metabolism mode of arsenic in saliva was similar with that in blood,suggested that salivary arsenic can also well reflect the exposure level of arsenic in the body.

关 键 词：亚砷酸盐类 砷 唾液 血液 

分 类 号：R994.6[医药卫生—毒理学] Q584[医药卫生—药学]