高效液相色谱法检测上海市两水厂原水和出厂水中神经类毒素-a污染水平  

Determination of anatoxin-a in raw and finished water from two water plants in Shanghai by high performance liquid chromatography

作　　者：张红梅[1] 付梓淳[1] 刘晓琳[1] 郑唯韡[1] 王霞[1] 虞聪聪[1] 蒋颂辉[1] 屈卫东[1] 

机构地区：[1]公共卫生安全教育部重点实验室复旦大学公共卫生学院环境卫生学教研室,上海200032

出　　处：《卫生研究》2012年第6期971-975,980,共6页Journal of Hygiene Research

基　　金：上海市科委社会发展领域重大科技项目(No.09DZ120010B);“十一五”科技支撑计划(No.2006BAI19B02);国家863高技术研究发展计划重点课题(No.2008AA062501)

摘　　要：目的建立高效液相色谱法(HPLC)检测饮用水中神经类毒素-a的方法。方法采集以青草沙水库为水源的A、B两水厂的原水和出厂水各1L,经GF/C滤膜过滤,弱阳离子交换(WCX)填料分别富集胞内和溶解性神经类毒素-a,同时,确定最佳的三氟乙酸(TFA)甲醇配比洗脱条件。采用HPLC定性定量分析以青草沙为水源的水厂原水和出厂水神经类毒素-a含量。结果 0.2%TFA甲醇洗脱效果最好。本研究建立的HPLC法检测水中神经类毒素-a,其标准曲线线性范围为0.01～5μg/ml,R2为0.9999,检出限2.9ng/L,加标回收率75.2%～100.6%,相对标准偏差(RSD%)6.38%～8.76%。连续6日加标检测均值与真实值一致,日内和日间RSD%在1.60%～8.90%之间。两水厂原水中均可检出神经类毒素-a,A、B水厂神经类毒素-a含量分别为0.028μg/L和0.137μg/L,出厂水中均未检出神经类毒素-a。结论本方法检出限低,回收率高,精密度高,稳定性好,可靠性强,可用于水中痕量神经类毒素-a检测,并可连续批量检测。为防止神经类毒素-a对人群健康的潜在危害,应加强监测青草沙库区及以此为水源的水厂原水和出厂水神经类毒素-a。Objective To establish the HPLC detection method for anatoxin-a in drinking water.Methods 1L water was collected,respectively,from raw and finished water in A and B water-plants provided source water by Qingcaosha Reservoir.Water samples were filtered by GF/C membrane,and then intracellular and dissolved anatoxin-a were enriched by weak cation-exchange（WCX） packing,respectively.The optimal ratio of trifluoroacetic acid（TFA） versus methanol for elution was determined.HPLC was employed for qualitative and quantitative analysis of anatoxin-a in water.Results Methanol containing 0.2% TFA had an optimal elution effect.The linear range of standard curve was 0.01-5μg/ml and R2 value was 0.9999.The detection limit of anatoxin-a was 2.9ng/L in water sample.The spiked recovery rates and the relative standard deviation（RSD%） were 75.2%-100.6% and 6.38%-8.76%,respectively.When detected continuously for six days,the average values were consistent with the true values,and RSD% ofintra-and inter-day was among 1.60%-8.90%.Anatoxin-a was detectable in raw water of both water-plants.0.028μg/L anatoxin-a of A water-plant and 0.137μg/L anatoxin-a of B water-plant.Anatoxin-a was un-detectable in finished water of both water-plants.Conclusion The current method can satisfy the detection of traces of anatoxin-a in drinking water and be used for continuous batch detection because of the low detection limit,high recovery rate and accuracy,good stability and reliability.More attention should be paid to surveillances of Qingcaosha Reservoir and raw and finished water from it,in order to prevent potential danger of Anatoxin-a on human health.

关 键 词：饮用水 神经类毒素-a 色谱检测法 

分 类 号：X524[环境科学与工程—环境工程] R123.1[医药卫生—环境卫生学]