孔雀草试管苗叶片AP1基因的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of AP1 Gene from the Leaves of in vitro Plantlets in Tagetes patula L.

作　　者：刘敏[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2012年第10期1778-1783,共6页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划(No.2007BAD07B03);福建省重大科技平台建设项目(No.2008N2001)

摘　　要：以孔雀草试管苗叶片为材料,成功克隆了孔雀草AP1基因的cDNA全长,并对其进行了生物信息学分析。AP1基因全长919 bp(GenBank登录号为JX310277),其中5′-UTR 92bp、3′-UTR 149bp、3′端poly(A)尾巴25 bp,开放阅读框为678 bp,编码225个氨基酸。AP1-1可能存在于细胞核中,为亲水蛋白,不含信号肽,共形成4个卷曲螺旋结构;二级结构主要有α螺旋和无规则卷曲构成,存在亮氨酸拉链结构和1个MADS-box区。此蛋白可能发生磷酸化位点的位置有7个。从系统进化树分析表明,该蛋白与甘菊具有较高的亲缘关系。In this experiment, the full-length cDNA of AP1 gene was successfully cloned from the leaves of in vitro plantlets and the bioinformatics analysis for AP1 gene was also conducted in Tagetes patula L. The full-length of AP1 gene was 919 bp （The accession number was JX310277 in GenBank）, containing 92 bp 5＇-UTR, 149 bp 3＇-UTR, and 3＇-end involved 25 bp poly （A） tails, the open reading frame had 678 bp, encoding 225 amino acids. AP1-1 protein was likely located in the cell nuclei, which was hydrophilic, without signal peptide and with 4 coil helix structures; The secondary structure was mainly constituted by the α-helix and random coil, and there existed a leucine zipper structure and a MADS-box domain. This protein had likely 7 phosphorylation sites. The phylogenetic tree analysis indicated that this protein was highly genetic relationship with chrysanthemum lavandulifolium.

关 键 词：孔雀草 试管苗 AP1基因 克隆 生物信息学分析 

分 类 号：S681.9[农业科学—观赏园艺]