胡椒根际溶磷真菌的分离、鉴定和生物学测定  

Isolation,Identification and Test of Biological Property of a Phosphorus-solubilizing Fungus in Rhizosphere of Piper nigrum L.

作　　者：郑肖兰[1] 贺春萍[1] 李锐[1] 崔昌华[2] 马小娟[2] 梁艳琼[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]海南大学环境与植物保护学院,海南海口570228

出　　处：《热带作物学报》2012年第10期1861-1868,共8页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.309016);海南省重点科技项目(No.090141)

摘　　要：利用无机磷液体培养基进行生物集富．采用系列稀释和平板涂布法成功地从正常生长的胡椒根际土壤分离筛选获得溶磷能力较强的真菌．命名菌株H3，其D／d值为1．67，有效溶磷量为400．28mg／L。通过形态学和分子生物学技术测定．该菌株鉴定为棘孢青霉菌（Penicillium aculeatum．Raper＆Fennell），该菌株的生物学特性研究表明．适宜溶磷能力的碳源是葡萄糖、果糖、蔗糖和麦芽糖，对氮源的要求不严格，供试13种氮源均为适宜氮源，适宜生长的培养基初始pH值为8，适宜生长的温度为26～37℃，3种光照方式对生长无显著影响，菌丝段经65℃10min处理仅有个别菌丝段存活。The biological concentration through inorganic phosphorus fluid nutrient medium was studied in this article. A fungus with high phosphate solubilizing ability by serial dilution and plate spreading was isolated from pepper rhizosphere soil. The D/d value of the strain named as H3 was 1.67. Effective phosphorous was 400.28 mg/L. Through morphological and molecular technology mensuration, the strain was identified as Penicillium aculeatum. Raper ＆ Fennell. The biological characteristics research indicated that the suitable carbon sources for phosphate solubilization were glucose, fructose, sucrose, maltose while not so strict with nitrogen source. The 13 kinds of nitrogen source on approval were all suitable, and the initial pH of optimal culture medium for growth was 8, temperature 26-37 ℃. None of the three illumination methods showed obvious effect on its growth, and only specific hvphal fragment was able to survive under the treatment of 65 ℃ for ten minutes.

关 键 词：溶磷真菌 棘孢青霉 生物学特性 

分 类 号：Q939.99[生物学—微生物学]