暑热宁合剂中葛根素HPLC测定条件的优化  

Optimization of HPLC Conditions of Puerarin in Summer Heat Ning Mixture

作　　者：吴娇[1] 李进[2] 李凤丽[2] 陈涛[2] 张元元[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《辽宁中医药大学学报》2012年第12期33-35,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家"重大新药创制"科技重大专项基金资助项目(2010ZX09102-201)

摘　　要：目的:优化暑热宁合剂中葛根素的高效液相色谱法测定条件。方法:色谱柱:Xterra188 Waters RP C18(250mm×4.6mm,5μm);检测器:Waters2998二级管阵列检测器(DAD);工作站:Waters Empower;流动相:乙腈-水(10:90);流速:1mL/min;检测波长:250nm。结果:该条件下可使暑热宁合剂中主要成分葛根素达到较好的分离,阴性无干扰。结论:该方法操作简便,结果准确可靠,可实现该样品的简便、准确、快速、经济实用的分析。Objective：To optimize the chromatographic conditions in determining Puerarin in Summer Heat Ning Mixture.Method：Xterra188 Waters RP C18（250mm×4.6mm,5μm）column;detector：waters2998;workstation：waters Empower.The motile phase：acetonitrile-water（10：90）,the flow rate was 1mL·min-1,and the detection wavelength was set at 250nm.Results：This condition can make the Puerarin achieve good separation.Conclusion：The proposed method was simple,accurate and precise;help to a simple,accurate,fast and inexpensive analysis of the adenosine phosphates.

关 键 词：高效液相色谱法 暑热宁合剂 葛根 葛根素 

分 类 号：R286.0[医药卫生—中药学]