黄河三角洲泥质沉积区表层沉积物古菌分布特征  

Diversity of achaea communities in mud wedge sediments from the Yellow River Delta

作　　者：王涛[1] 萨仁高娃[2] 李铁刚[2] 马学恩[1] 春梅[1] 于心科[2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]中国科学院海洋研究所,海洋地质与环境重点实验室,山东青岛266071

出　　处：《海洋科学》2012年第10期5-12,共8页Marine Sciences

基　　金：中国科学院知识创新工程重要方向项目(KZCX2-YW-JC201)

摘　　要：通过古菌16S rDNA基因文库技术,在黄河三角洲泥质沉积区6个站位共获得568个克隆,经处理得到73个OUTs(Operational Taxonomic Units),在此基础上进行系统进化树以及统计学分析。结果显示,在本次研究的6个站位的表层沉积物中,古菌序列均来自于泉古菌(Crenarchaeota)和广古菌(Euryarchaeota):其中以Marine Crenarchaeotic Group I(MGI)为主,仅含少量的Miscellaneous Crenar-chaeotic Group(MCG),Marine Benthic Group B(MBGB)等其他种属。研究结果表明,6个不同站位的沉积物中只有5个古菌类群,群落分布较为均匀,多样性不高。研究结果揭示了黄河三角洲表层沉积物中古菌横向分布特征,并为今后在黄海区域开展古菌生态学研究提供了数据信息,特别是在当前古菌研究主要集中在深海区域的背景下,对相应的近海区域古菌研究具有一定的参考意义。Archaeal 16S rDNA clone libraries were constructed from sediments of different location to elucidate the distribution and diversity of archaea in the Yellow River Delta coastal mud wedge of the Bohai Sea and the Yellow Sea. A total of 568 archaeal 16S rDNA clones were examined and a total of 73 OTUs were obtained. Phylogenetic and statistic analysis showed that the archaeal diversity in the collected samples was low. 16S rDNA sequences are within phylums of Crenarchaeota and Euryarchaeota, respectively. The majority of archaeal phylotypes were Ma- rine Crenarchaeotic Group I（MGI）, others included Miscellaneous Crenarchaeotic Group （MCG）, Marine Benthic Group B （MBGB）, et al. This research has provided very important information, which is very helpful to our under- standing archaeal community and environment of the Yellow River Delta.

关 键 词：黄河三角洲 表层沉积物 古菌 多样性 

分 类 号：Q16[生物学—普通生物学]