巴戟天寡糖对海马神经细胞再生及神经元生长的影响  

Effect of Morinda officinalis how oligosaccharides on the regeneration and growth of hippocampal neurons

作　　者：邹连勇[1] 马远林[1] 宓为峰[1] 李玲芝[1] 张鸿燕[1] 

机构地区：[1]北京大学精神卫生研究所,卫生部精神卫生学重点实验室,北京100191

出　　处：《中国新药杂志》2012年第22期2623-2626,共4页Chinese Journal of New Drugs

基　　金：国家“重大新药创制”科技重大专项(2008ZX09101)

摘　　要：目的:考察巴戟天寡糖对海马神经细胞再生及神经元生长的影响。方法:成年ICR小鼠随机分为生理盐水对照组、氟西汀对照组(18 mg.kg-1)和巴戟天寡糖高、低剂量组(50,25 mg.kg-1),各组连续腹腔注射14 d。采用免疫组化法测定小鼠海马齿状回新增殖神经细胞的数目;通过原代细胞培养观察不同剂量巴戟天寡糖(1.25,2.5,5,10 mg.mL-1)对大鼠海马神经元树突生长的影响。结果:巴戟天寡糖50mg.kg-1能够显著促进成年小鼠海马神经细胞的再生;10 mg.mL-1巴戟天寡糖能够增加原代培养的海马神经元树突及分支数目(P<0.05)。结论:巴戟天寡糖对海马神经可塑性具有调节作用,该作用是否是其抗抑郁作用机制之一仍需进一步证实。Objective： To explore the effect of Morinda officinalis how oligosaccharides （MOs） on the regeneration and growth of hippocampal neurons. Methods： Adult ICR mice were randomly divided groups, and intraperitoneally injected with saline （ control）, fluoxetine （ 18 mg· kg^-1） and MOs （50, 25 mg·kg^-1 ） for 14 days. Immunohistochemistry was used to determine the proliferation of neural cells in hippocampus dentate gyrus. The effect of MOs at various concentrations （ 1.25, 2.5, 5 and 10 mg·mL^-1 ） on the growth of neuron dendrites was evaluated in the primary neuron culture. Results ： MOs 50 mg. kg^- 1 promoted the proliferation of neural cells in hip- pocampal dentate gyrus in vivo. MOs 10 mg·mL^-1 increased the number of dendrites and its branch of hippocampal neurons in vitro. Conclusion： MOs can regulate neural plasticity, including regeneration and growth of neurons, but further research is required to prove whether this effect is one of its antidepressant mechanisms.

关 键 词：巴戟天寡糖 抗抑郁药 海马 可塑性 

分 类 号：R961[医药卫生—药理学]