奥扎格雷纳米结构脂质载体包封率的测定方法  

Determination of entrapment efficiency of ozagrel-loaded nanostructured lipid carriers

作　　者：王坤[1] 杨磊[1] 王艳[1] 邱立朋[1] 叶慧珠[1] 刘玉卓[1] 王东凯[1] 

机构地区：[1]沈阳药科大学药剂学系,沈阳110016

出　　处：《中国新药杂志》2012年第22期2693-2696,共4页Chinese Journal of New Drugs

摘　　要：目的:建立奥扎格雷纳米结构脂质载体(ozagrel-loaded nanostructured lipid carriers,OZ-NLC)包封率的测定方法。方法:分别采用超滤法、葡聚糖凝胶柱层析法、超速离心法分离游离药物,以紫外分光光度法测定奥扎格雷的含量,计算包封率。结果:超滤法和葡聚糖凝胶柱层析法可有效分离OZ-NLC与游离药物,两种方法的平均回收率分别为98.31%和97.15%,平均包封率为59.21%和61.11%。结论:葡聚糖凝胶柱层析法操作简便,结果可靠,重现性好,故选择该方法测定OZ-NLC的包封率。Objective： To establish a method for determining the entrapment efficiency of ozagrel-loaded nanostructured lipid carriers （OZ-NLC）. Methods： The OZ-NLC and OZ were separated by uhrafiltration, Sephadex G-50 column chromatography and ultracentrifugation. The UV spectrophotometry was employed to determine the ozagrel content and entrapment efficiency. Results： OZ-NLC was effectively separated from free drugs by uhrafiltration and Sephadex G-50 column chromatography. The average recovery of uhrafihration and Sephadex G-50 chroma- tography was 98.31% and 97.15% , respectively. The average entrapment efficiency was 59.21% and 61.11% , respectively. Conclusion： Sephadex G-50 chromatography is simple, reliable and reproducible. This can be used for determining the entrapment efficiency of OZ-NLC.

关 键 词：奥扎格雷 纳米结构脂质载体 包封率 超滤法 葡聚糖凝胶柱层析法 超速离心法 

分 类 号：R943[医药卫生—药剂学] R972.7[医药卫生—药学]