反相离子对色谱法测定复方维生素片中3种水溶性维生素的含量  

Reversed-phase ion-pair chromatography method for the determination of three water soluble vitamins in compound vitamin tablets

作　　者：贺霞[1] 史朝晖 刘慧[1] 张小岭[1] 闫笑笑[1] 周晓星[1] 王东凯[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]海南碧凯药物研究所有限公司,海口570216

出　　处：《中国新药杂志》2012年第22期2697-2700,共4页Chinese Journal of New Drugs

摘　　要：目的:建立反相离子对色谱法测定复方维生素片中维生素B1、维生素B2、维生素B6含量的方法。方法:以己烷磺酸钠作为离子对试剂,采用Diamonsil C18(250 mm×4.6 mm,5μm)柱,同时测定复方维生素片中维生素B1、B2和B6的含量。结果:维生素B1、B2和B6分别在6.3～14.7μg.mL-1(r=0.999 8),7.2～16.8μg.mL-1(r=0.999 5),6.3～14.7μg.mL-1(r=0.999 6)范围内线性关系良好,平均回收率分别为100.08%(RSD=0.84%,n=9),100.35%(RSD=1.26%,n=9),99.41%(RSD=1.10%,n=9)。结论:本方法快速、简便、准确,分离效果好,辅料无干扰,可用于该类制剂中维生素B1,B2和B6的含量测定及质量控制研究。Objective： To establish an ion-pair reversed-phase chromatographic method for the determination of vitamin B1 , B2 and B6 in compound vitamin tablets. Methods： The contents of vitamin B1 , B2 and B6 were simultaneously determined by ion-pair reversed-phase high performance liquid chromatography. Results： The linear ranges of vitamin B1, B2 and B6 were 6.3 - 14.7 μg· mL^ -1 （ r = 0. 999 60 ）, 7.2 - 16.8μg· mL^ -1 （ r = 0. 999 1 ） and 6.3 - 14.7 μg· mL^ -1（r =0. 999 2） with the average recoveries of 100.08% （RSD =0.84%, n =9）, 100.35% （ RSD = 1.26% , n = 9） and 99.41% （ RSD = 1.10% , n = 9）, respectively. Conclusion ： The method is simple, rapid, accurate and reliable, and can be used for the assay and quality control of compound vitamin tablets.

关 键 词：维生素B1 维生素B2 维生素B6 含量测定 反相离子对色谱法 

分 类 号：R927.2[医药卫生—药学] R977.2