人参皂苷Rg1对大鼠海马神经元缺糖氧／复糖氧后钙内流的影响  

Effect of ginsenoside Rgl on calcium overload induced by oxygen - glucose deprivation and reperfusion in cultured hippocampal cells

作　　者：陈彦[1] 吴鸿浩[1] 何斌[1] 陈旭峰[1] 吕金如[1] 王淦楠[1] 吴昊[1] 张劲松[1] 汪琴[2] 吴婷[2] 

机构地区：[1]南京医科大学第一附属医院急诊中心,江苏南京210029 [2]南京医科大学第一附属医院神经内科,江苏南京210029

出　　处：《中国急救医学》2012年第11期1001-1004,I0001,共5页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目（30901578）;江苏高校优势学科建设工程项目（Jx10231081）;江苏省卫生厅“科教兴卫工程”开放课题（XK20200905）

摘　　要：目的观察人参皂苷Rg1对大鼠海马神经元缺糖氧／复糖氧后钙内流的影响，并探讨其可能的脑保护机制。方法建立大鼠海马神经元缺糖影复糖氧模型，随机分为正常对照组、模型组和人参皂苷Rg1干预组（5、20、60μmol／L）。复糖氧后24h以Fluo-3 AM荧光染色法观察各组海马神经元细胞内钙离子浓度变化，以Hoechst染色法检测细胞凋亡，并检测细胞四甲基偶氮唑盐（MTT）代谢率。结果与模型组比较，人参皂苷Rg1中、高剂量组海马神经元细胞内钙离子浓度降低，凋亡细胞减少，MTT代谢率升高，人参皂苷Rg1低剂量组变化不明显。结论脑缺血后神经元细胞内钙超载与脑损伤关系密切，人参皂苷Rg1可通过减少缺糖氧神经元细胞内钙内流，发挥脑保护作用。Objective To observe the effect of ginsenoside Rgl on calcium overload induced by oxygen -glucose deprivation and reperfusion injury in cultured hippocampal cells and to study its possible mechanisms. Methods The rat models of oxygen - glucose deprivation and reperfusion of hippocampal neurons were build, and were randomly divided into control group, model group and ginsenoside Rgl treatment groups（5,20,60 μmol/L）. At 24 h after reperfusion, the intracellular levels of calcium was detected by Fluo -3 AM staining, the cell apoptosis was evaluated by Hochest staining, and the metabolic rate of MTT were determined. Results Compared with model group,the intracellular levels of calcium and cell apoptosis were decreased, and the metabolic rate of MTT were improved in the groups receiving ginsenoside Rgl middle and high dose except low dose. Conclusion Ginsenoside Rgl has neuroprotective effect by decreasing the intracellular levels of calcium after cerebral ischemia.

关 键 词：脑缺血 人参皂苷RG1 钙超载 细胞凋亡 海马神经元 

分 类 号：R286.0[医药卫生—中药学]