沙冬青细胞对MeJA处理的初始生理响应  

Primary Physiological Responses of Ammopiptanthus mongolicus Cells Induced by Methyl Jasmonate

作　　者：高海波[1,2] 

机构地区：[1]临沂大学生命科学学院,临沂276005 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《林业科学》2012年第10期24-29,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30871727);长江学者与创新团队发展计划项目(PCSIRT0607)

摘　　要：研究沙冬青细胞经茉莉酸甲酯(MeJA)瞬时处理后发生的Ca2+离子流、H2O2含量及质膜电位的变化情况。结果表明:MeJA引起沙冬青细胞H+质子内流、H2O2积累及膜电位去极化。胞内钙库抑制剂钌红预处理细胞后,抑制了MeJA处理引起的H+质子内流、H2O2的积累及膜电位去极化;表明MeJA瞬时处理后,胞内钙库中Ca2+的释放是细胞随后产生的响应。H2O2清除剂预处理彻底抑制MeJA引起的H+质子内流及质膜电位的去极化反应,证明H2O2的积累位于H+质子内流的上游,H+质子内流是沙冬青细胞膜电位去极化的主要原因。This study investigated changes in Ca2＋ flux,hydrogen peroxide content and membrane potential of Ammopiptanthus mongolicus cells in response to methyl jasmonate treatment.Results showed that methyl jasmonate caused proton influx,hydrogen peroxide production and membrane depolarization,which were completely inhibited by pretreatment with intracellular calcium store release inhibitor ruthenium red,demonstrating that calcium release from intracellular calcium store was necessary for proton influx,hydrogen peroxide production and membrane depolarization.Both proton influx and membrane depolarization,triggered by treatment with methyl jasmonate,were inhibited by pretreatment with a hydrogen peroxide scavenger,indicating that hydrogen peroxide production was also necessary for proton influx which was the main reason of membrane depolarization.

关 键 词：茉莉酸甲酯 沙冬青 Ca2+离子流 H2O2 膜电位 

分 类 号：Q942.5[生物学—植物学] S718.43[农业科学—林学]