强直性脊柱炎滑膜细胞培养上清液对韧带成纤维细胞成骨分化的影响  

Effects of the supernatant from ankylosing spondylitis synovial cells culture on the osteogenic differentiation of ligament fibroblasts

作　　者：徐卫东[1] 梁志民[1] 袁恒锋[1] 

机构地区：[1]第二军医大学附属长海医院骨科,上海200433

出　　处：《中华关节外科杂志（电子版）》2012年第5期57-60,共4页Chinese Journal of Joint Surgery(Electronic Edition)

摘　　要：目的探讨强直性脊柱炎(AS)滑膜细胞培养上清液对韧带成纤维细胞成骨分化的影响。方法分别进行AS患者滑膜和韧带成纤维细胞原代培养,收集第1代的滑膜细胞培养液上清液,应用此上清液对韧带成纤维细胞进行诱导,分别于0、5、10、15、20d检测碱性磷酸酶和骨钙素表达水平,并于25d时进行钙结节染色。结果在应用AS滑膜细胞培养上清液对韧带成纤维细胞进行诱导的第10天,碱性磷酸酶(ALP)和骨钙素(OC)表达水平明显升高,并在第15天达到高峰。第25天时进行钙结节VonKossa染色,发现有钙结节形成。结论 AS滑膜细胞培养上清液对韧带成纤维细胞具有成骨诱导分化的能力。Objective To investigate the effects of synovial cells culture fluid on the differentiation of ligament fibroblasts into osteoblasts. Methods Specimens of ligament and synovium were obtained from the hip joints with ankylosing spondylitis respectively. The primary cell culture of ligament fibroblasts and synovial cells were processed and then the supernatant of synovial cells in the first passage was collected and used to induce ligament cells. Osteogenic phenotype and alkaline phosphatase （ALP） activity were determined at the days of 0, 5,10, 15 and 20 respectively. Calcium deposit was detected by Von Kossa staining at the 25th day. Results With time extending, the expression of ALP and osteocalcin increased significantly at day 10 and reached a peak at day 15. Calcium deposit could be detected at day 25. Conclusions The supernatant of synovial cells cuture plays a certain role on the differentiation of ligament fibroblasts into osteoblasts.

关 键 词：脊柱炎 强直性 滑膜 韧带 成纤维细胞 

分 类 号：R593.23[医药卫生—内科学]