基于荧光定量PCR的兔毛、狐狸毛纤维定性检测方法  

Qualitative analyzing method based on real-time PCR for identifying rabbit fiber and fox fiber

作　　者：费静[1] 董正廉[1] 周辉[1] 陆维民[1] 杨娟[1] 唐敏峰[1] 

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《上海纺织科技》2012年第11期56-58,共3页Shanghai Textile Science & Technology

基　　金：国家质检总局科技项目(2011IK103)

摘　　要：通过设计针对兔、狐线粒体12SrRNA基因的特异性TaqMan探针和引物对,建立了基于DNA分析的兔毛纤维和狐狸毛纤维鉴别方法。该方法特异性好,且灵敏度达到了1%,除定性分析纤维的种类外,还可以有效鉴别微量的异纤维。A DNA based method of identifying fiber fox fiber has been through designing sets of species-specific TaqMan probes and primers on mitochondrial 12SrRNA gene. The method features species specific and high sensitivity （1%）. Besides being used for qualitative distinguish of fox fiber and rabbit fiber, it is also an effective way to identify mixed foreign fibers of micro amount.

关 键 词：兔毛纤维 狐狸毛纤维 DNA 物种鉴定 

分 类 号：TS101.921[轻工技术与工程—纺织工程] O657.3[轻工技术与工程—纺织科学与工程]