橡胶树胶乳死皮相关蛋白的鉴定及分析  

Identification and Analysis of Latex Proteins Related to Tapping Panel Dryness in Hevea brasiliensis

作　　者：袁坤[1] 徐智娟[1] 王真辉[1] 杨礼富[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737

出　　处：《西北林学院学报》2012年第6期105-109,127,共6页Journal of Northwest Forestry University

基　　金：中央级公益性科研院所基本科研业务费专项(YWFZX2008-12、2009-12、2010-9);海南省重点科技项目(90107);现代农业产业技术体系建设专项资金(CARS-34-02A)

摘　　要：橡胶树死皮(tapping panel dryness,TPD)是一种复杂的生理综合症,严重制约了天然橡胶产量的提高。为了分离鉴定橡胶树胶乳中与死皮发生密切相关的蛋白,采用双向电泳技术(2-DE)比较了其健康树和死皮树胶乳蛋白质组的差异。结果显示,在健康树和死皮树蛋白图谱上分别检测到1 158±25和1 173±30个蛋白点,其中54个蛋白点差异表达。经基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)分析和数据库检索后,成功鉴定16个蛋白,这些蛋白中的3-磷酸甘油醛脱氢酶、紫色酸性磷酸酶、动力相关蛋白、烯醇酶以及小橡胶粒子蛋白等可能在死皮发生过程中具有关键作用。为从分子水平上阐明橡胶树死皮发生机制提供了一定的理论依据。Tapping panel dryness （TPD） in Hevea brasiliensis is a complex physiological syndrome and it seriously limits the increase of natural rubber production. In order to separate and identify the proteins closely correlated with TPD in H. brasiliensis, the proteomes from healthy and TPD trees were compared by 2-DE. 1 158±25 and 1 173±30 protein spots were observed in the protein profiles of healthy and TPD trees respectively. Among them, 54 protein spots were expressed differentially. Subjected to MALDI- TOF-MS and database searching, 16 proteins were identified successfully. Some of them, such as the glyc- eraldehyde-3-phosphate dehydrogenase （GAPDH）, purple acid phosphatase （PAP）, dynamin-related pro- tein （DRP）, enolase and small rubber particle protein （SRPP）, might play key roles in the process of TPD occurance. The current study will provide theoretical bases for further elucidating the mechanism underly- ing TPD in H. brasiliensis at the molecular level.

关 键 词：橡胶树 死皮 双向电泳 质谱 蛋白质组 

分 类 号：S796[农业科学—林木遗传育种]