不同方法提取蚱蝉若虫蛋白质效果比较  

Comparison of Chemical Extraction Methods of the Proteins from the Larva of Cryptotympana pustulata

作　　者：金会鑫[1] 谢寿安[1] 史睿杰[1] 汤耀宗[1] 王翻艳[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2012年第6期128-130,138,共4页Journal of Northwest Forestry University

基　　金：国家级大学生创新性试验计划项目(101071213)

摘　　要：以蚱蝉(Cryptotympana pustulata)若虫为材料,采用正交试验法,对蚱蝉若虫蛋白质的提取条件进行了研究。结果表明,碱提蛋白法的蛋白质得率最高,其次为盐提蛋白法,Tris-HCl提蛋白法得率最低。碱提蛋白法的主要工艺指标为:碱液质量分数0.5%、温度60℃、时间60min、固液体积比为1∶5时蛋白质得率有最大值;盐提蛋白法的主要工艺指标为:盐液质量分数1.5%、温度40℃、时间120min、固液体积比为1∶10时蛋白质得率有最大值;Tris-HCl提蛋白法的主要工艺指标为:Tris-HCl液pH值8.1、抽提温度20℃、抽提时间30min、固液体积比1∶10时蛋白质得率有最大值。The extraction methods of the protein from the larva of Cryptotympana pustulata was investiga- ted by the orthogonal test. The results showed that alkaline extraction method had the highest yield, fol- lowed by salt extraction and Tris-HCl extraction. The optimum extraction conditions for alkaline method with the highest protein extraction yield were obtained as alkali concentration 0.5%, temperature 60℃, extraction time 60 min, and the ratio of solid to liquid 1 ： 5. The optimal conditions for salt method were obtained as salt concentration 1.5%, temperature 40℃, extraction time 120 min, and the ratio of solid to liquid 1 ~ 10. The best conditions of Tris-HC1 method were obtained as pH 8.1, temperature 20℃, extrac- tion time 30 min, and the ratio of solid to liquid 1 ： 10.

关 键 词：蚱蝉若虫 蛋白质提取方法 正交试验 

分 类 号：S759.8[农业科学—森林经理学]