不同来源牛体外胚胎与牛子宫内膜细胞共培养体系的建立  

Establishing an Effective Way to Co-culture the in-vitro Bovine Blastocysts from Different Methods With Their Uterine Endometrium Cells

作　　者：张彧[1,2] 陈利平[1] 梁鸿斌[2] 李娅杰[1] 赵雅琦[1] 阎芃[1] 杨菲[1] 倪和民[1] 郭勇[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京奶牛中心,北京100192

出　　处：《中国农学通报》2012年第29期39-43,共5页Chinese Agricultural Science Bulletin

基　　金：自然科学基金"IFNτ协调Galectin15对绵羊体外胚胎着床调节机制的研究"(30871836)

摘　　要：为了初步建立有效的牛胚胎与子宫内膜细胞的体外共培养体系。本实验分别将体外受精第7天后的胚胎和孤雌激活,与培养7天的胚胎与子宫内膜细胞共培养,在48、72h后分别统计体外受精实验组和孤雌激活实验组的孵化率。实验结果表明:在本实验室培养条件下,在与子宫内膜细胞共培养48h后,体外受精胚胎和孤雌发育胚胎在孵化率上存在显著差异(40±5)%、(25±5)%,(P<0.05);与子宫内膜共培养72h后,孵化率无显著差异(70±8.66)%,(65±5)%,(P>0.05)。本实验证明了在体外环境下,牛体外胚胎与子宫内膜细胞共培养后能够正常孵化,可以初步建立起牛体外胚胎与子宫内膜细胞的体外共培养体系,并为进一步研究该体系对牛体外胚胎培养质量的影响奠定一些基础。In this study,it was initiated to establish an effective way to co-culture the bovine blastocysts and their uterine endometrium cells in vitro.Here,total 7 days in vitro fertilized（IVF） or 7 days parthenogenetic（PA） bovine blastocysts were put into the quadripuntal broad with 42,72 h uterine endometrium cells.The results showed that,after being co-cultured with the uterine endometrium cells for 48 h,the hatching rate of IVF blastocysts was significantly higher than that of PA group（40±5）% vs（25±5）%,P 0.05）,but after being co-cultured for 72 h,there was no significant differences of hatching rate between them（70 ± 8.66）% vs（65±5）%,P0.05）.In conclusion,these results suggested that,such kind of in vitro co-culture system between those in vitro produced bovine blastocysts and the uterine endometrium cells,be a potential and effective way for further investigating the quality of bovine embryos.

关 键 词：牛体外胚胎 子宫内膜细胞 体外共培养 

分 类 号：S814.8[农业科学—畜牧学]