银翘天甘对猪链球菌Ⅱ型病理模型的防治试验  

Prevention and Treatment of Yinqiaotiangan against Pathologic Model for Streptococcus suis SerotypeⅡ in Kunming Mice

作　　者：蒋红[1] 王宏军[1] 周铁忠[1] 张利勃[1] 刘锴[2] 张爽[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]内蒙古民族大学,内蒙古通辽028000

出　　处：《中国农学通报》2012年第29期44-48,共5页Chinese Agricultural Science Bulletin

基　　金：辽宁省教育厅资助项目"加减银翘散中药制剂的药效学研究"(L2010256);辽宁省农业科技攻关重点项目"辽宁省重大动物疫病病原变异与综合防控技术研究"(2011214001)

摘　　要：为观察银翘天甘中药制剂体内抗猪链球菌病的效果。用猪链球菌Ⅱ型对18～22g昆明鼠攻毒并开展预防和治疗实验。预防试验是用猪链球菌Ⅱ型对18～22g昆明鼠攻毒同时口服银翘天甘中药制剂0.5、1.0、2.0g/mL,1次/d,连用3天,观察7天后的动物发病率、死亡率和保护率;治疗试验是先用猪链球菌Ⅱ型攻毒并建立昆明鼠疾病模型后口服上述药物,2次/d,连用3天,观察7天后的动物死亡率、治愈率和有效率。预防试验结果表明:银翘天甘中药制剂组实验动物死亡率均极显著低于感染对照组(P<0.01);给药组实验动物发病率均极显著低于感染对照组(P<0.01);治疗试验结果表明:银翘天甘中药制剂组昆明鼠死亡率极显著低于阴性对照组(P<0.01);低剂量组治愈率极显著高于中、高剂量组(P<0.01),低剂量组有效率显著高于中、高剂量组(P<0.05),与阳性对照组之间无显著性差异(P>0.05)。由此表明,口服低剂量银翘天甘中药制剂能够在昆明鼠猪链球菌病理模型上发挥预防和治疗作用。In order to investigate antimicrobial effect to Streptococcus suis serotype Ⅱ on Chinese herbal preparation of Yinqiaotiangan in vivo.The prevention and treatment test was carried with Kunming mice about 18-22 g weight.In the prevention trial these mice were attacked by the Streptococcus suis serotype Ⅱ,at the same time,taken orally 0.5 g/mL,1.0 g/mL and 2.0 g/mL Chinese herbal preparation of Yinqiaotiangan respectively.And then the incidence,mortality,and protective rate were detected after 7 days when the Chinese herbal preparation was continuous used 3 d,once a day.In the treatment test,the Streptococcus suis serotype Ⅱ disease model was established in Kunming mice.Then these mice were taken orally 0.5 g/mL,1.0 g/mL and 2.0 g/mL Chinese herbal preparation of Yinqiaotiangan respectively.The cure rate,effective rate and incidence were detected after 7 days when the preparation of Yinqiaotiangan was continuous used 3 d,twice a day.The prevention trial results showed that the protective rate in experimental groups were significantly higher than those in control（P0.01）,the incidence and mortality were significantly lower than that of the control（P0.01）.The treatment test results showed that the incidence of the Chinese herbal preparation groups was significantly lower than that of control group（P0.01）,the cure rate of low dose group were higher than that of middle and high dose group（P0.01）,and the effective rate of low dose group was significantly higher than that of middle and high dose group（P0.05）.So the pathologic model of Streptococcus suis serotype Ⅱ could be effectively prevented and treated by oral the low dose Yinqiaotiangan preparation in Kunming mice.

关 键 词：银翘天甘 猪链球菌Ⅱ型 病理模型 昆明鼠 

分 类 号：S853.74[农业科学—临床兽医学]