正交试验法优化催情助孕液制备工艺  

Optimization of Preparation Technology for the Cuiqingzhuyunye Liquid by Orthogonal Experiment Design

作　　者：王东升[1] 张世栋[1] 李世宏[1] 苗小楼[1] 尚小飞[1] 严作廷[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省中兽药工程技术研究中心,兰州730050

出　　处：《中国农学通报》2012年第29期75-78,共4页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划项目"奶牛不孕症防治药物研究与开发"(2012BAD12B03-1-1);兰州市科技计划项目"中药清宫助孕液的研制与推广"(2010-1-219)

摘　　要：为了优化催情助孕液的水提醇沉工艺,采用正交试验法设计,用高效液相色谱法测定制剂中淫羊藿苷含量并以其为评价指标,考察了加水量、煎煮时间、煎煮次数、醇沉浓度、醇沉次数对制剂工艺的影响。结果表明:最佳制剂工艺为加10倍量水,煎煮3次,每次1h,乙醇醇沉浓度为75%,醇沉1次。用该工艺制备的催情助孕液淫羊藿苷含量为0.98892mg/mL,说明该优选工艺稳定可行。To optimize preparation technology of the water extract and alcohol precipitation for Cuiqingzhuyunye liquid,volume of water,the time and frequency of decoction,the concentration and frequency of alcohol precipitation were investigated with the content of the icariin as an indicator,by orthogonal design.The results showed that the optimum technology was as follows,extracted for 3 times with water 10 times of the amount of the crude drug and 1 hour every time,then alcohol precipitation for 1 time by using 75% alcohol.The content of the icariin in Cuiqingzhuyunye liquid was 0.98892 mg/mL,which indicated that the optimum technology was stable and feasible.

关 键 词：淫羊藿苷 正交设计 HPLC 含量 

分 类 号：S853.73[农业科学—临床兽医学]