西太公鱼微卫星的筛选与分析  

Isolation and characterization microsatellite markers from Hypomesus nipponensis

作　　者：龚小玲[1] 吴颖[1] 任圣杰[1] 刘其根[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2012年第6期951-954,共4页Journal of Shanghai Ocean University

基　　金：上海市科技攻关项目(10dz1200804);上海市自然科学基金(09ZR1421700);上海市教育委员会创新项目(09YZ279);上海市重点学科建设项目(S30701)

摘　　要：利用磁珠富集法从西太公鱼(Hypomesus nipponensis)基因组中筛选到14对多态性微卫星引物,并对来自黑龙江镜泊湖的西太公鱼24个样本进行检测:扩增的等位基因数在3～25个之间,平均为8.4个;观察杂合度(HO)和期望杂合度(HE)分别为0.266 7～1.000 0和0.282 5～0.9322;多态信息含量(PIC)为0.239 2～0.910 6;位点HN-075和位点HN-250显著偏离哈迪-温伯格平衡(P<0.05),这些多态性微卫星位点可用于公鱼属鱼类种群遗传结构及其相关研究。Fourteen polymorphic microsatellite loci were isolated from the Japanese smelt（Hypomesus nipponensis）.These microsatellite markers were tested in a sample of 24 individuals.The number of alleles ranged from 3 to 25,with an average of 8.4 alleles per locus.The observed and expected heterozygosity of these loci varied from 0.266 7 to 1.000 0 and 0.282 5 to 0.932 2,respectively.Locus HN-075 and HN-250 deviated significantly from Hardy-Weinberg equilibrium（P0.05）.No genotypic disequilibrium among loci was found（P0.05）.These microsatellite markers could be useful in studing population structure of the Japanese smelt,or introduction routes of this fish to China.

关 键 词：西太公鱼 微卫星 种群 移植 

分 类 号：S917[农业科学—水产科学]