养殖菱斑龟肝病组织病理研究  

Histopathological study of hepatopathy in four diamondback terrapins(Malaclemys terrapin)

作　　者：雷洁[1] 王伟喆[1] 潘连德[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2012年第6期989-995,共7页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(39970582);上海市教育委员会重点项目(07zz135);上海市重点学科建设项目(Y1101);上海市高校知识服务平台项目(zF1206)

摘　　要：研究海口市泓旺农业养殖有限公司特种龟鳖养殖场养殖菱斑龟种龟发病的病例(4个),采用病理解剖和组织切片H.E染色,进行组织病理学研究。病理解剖显示,4只病龟的肝脏为病变最严重器官,出现色深及表面分布白色斑块的病理解剖症状;血涂片显示外周血液中的总白细胞比例及嗜酸性粒细胞比例明显增高;组织切片中可见肝组织病变最严重并具一致性,病龟C、D肝实质细胞普遍出现颗粒变性、以嗜酸性粒细胞为主的肝组织炎性细胞浸润,血铁黄质沉积,病龟B肝静脉扩张、分支血液回流受阻,肝窦间隙扩张淤血,部分肝细胞转化为空泡变性,病龟A肝实质细胞坏死、肝细胞索消失。4只病龟肝脏表面还出现局部纤维化坏死,眼睑组织静脉周围也出现嗜酸性粒细胞为主的炎性细胞浸润。根据病理解剖和组织病理检验结果,并结合对本场菱斑龟养殖情况的调查,认为该病为肝病,并伴有眼症状,其他器官组织病变属肝病的相伴症状。病龟由于肝功能逐渐衰竭而致死。Four diagnostically challenging cases of unknown hepatopathy in four female diamondback terrapins（Malaclemys terrapin） are reviewed and important differential diagnoses discussed.The methods are necropsy,blood smear and histopathological section.At necropsy,the livers were dark,atrophied,and the capsule fibrotic.Abnormalities noted in the hemogram are eosinophilia.The number of leukocytes and eosinophils was significantly higher than normal ranges for healthy reptiles.The pathological changes of livers are the most serious and have consistency.Granular degeneration and vacuolar degeneration happened in most hepatic parenchyma cells,and seriously even necrosis.The hepatic veins lined by inflammatory cells and hepatic congestion.At the end,the most hepatic parenchyma cells were necrosis except the portal area.The lesions in eyelids,lung and kidney are mild and not enough to threaten life,and were complications.The lesion in the four terrapins was diagnosed on these results as a hepatopathy,and the terrapins died of liver failure.There is no evidence to prove that is a parasite infection.

关 键 词：菱斑龟 肝病 组织病理 

分 类 号：S947.1[农业科学—水产养殖]