亚洲玉米螟piggyBac类似因子的克隆及序列分析  

Cloning and sequencing of piggyBac-like element in the Asian corn borer,Ostrinia furnacalis

作　　者：刘丹[1,2,3] 严善春[1] 谭安江[2,3] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040 [2]中国科学院上海生命科学研究院植物生理生态研究所昆虫科学研究中心 [3]中国科学院昆虫发育与进化生物学重点实验室,上海200032

出　　处：《南京农业大学学报》2012年第6期35-43,共9页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金重点项目(31030060)

摘　　要：为了深入了解piggyBac转座子家族的生物学特性及分子进化特点,采用简并PCR、反向PCR、RT-PCR和RACE等方法在农业害虫亚洲玉米螟中克隆到2个piggyBac类似因子,分别命名为OfPLE1和OfPLE2。利用生物信息学软件分析Of-PLE基因编码区序列结构以及与其他物种的系统发育关系,结果表明,OfPLE1全长1 866 bp,编码607个氨基酸;OfPLE2全长1 724 bp,编码434个氨基酸。两者同源性高达70%,与粉纹夜蛾piggyBac同源性分别为32%和26%,亲缘关系较远。The piggyBac transposon which was identified from the cabbage looper Trichoplusia ni,is a highly useful transposon in insect genetic manipulation.To further understand the biological and evolutionary characteristics of piggyBac-like elements（PLEs）in insects,two PLE sequences,named as OfPLE1 and OfPLE2,were cloned from the Asian corn borer based on degenerate PCR,inverse PCR,RT-PCR and RACE.The sequence structure of OfPLE gene coding region and the phylogenetic relationship with other species were analyzed by application of the bioinformatics software.The results showed that OfPLE1 was 1 866 bp long encoding 607amino acid residues and OfPLE2 was 1 724 bp long encoding 434 amino acid residues.Furthermore,they shared 70% homology with each other,and had 32% identity and 26% identity with Trichoplusia ni piggyBac transposon.

关 键 词：亚洲玉米螟 PIGGYBAC 克隆 序列分析 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]